UPLC-UV法分离分析橄榄叶及生物样本中的羟基酪醇  

An UPLC method for determination of hydroxytyrosol in extraction of olive leaf and several biosamples with UV detection

作　　者：冯晗珂 崔玉婷 毕金淼 米捷 高月华 陈杰巃 龙建纲[1] 李华 孔宇[1] FENG Hanke;CUI Yuting;BI Jinmiao;MI Jie;GAO Yuehua;CHEN Jielong;LONG Jiangang;LI Hua;KONG Yu(School of Life Science and Technology,Experimental Teaching Demonstration Center of Life Science,Xi’an Jiaotong University,Xi’an 710049)

机构地区：[1]西安交通大学生命科学与技术学院陕西省生命科学实验教学中心,西安710049

出　　处：《分析试验室》2020年第7期800-805,共6页Chinese Journal of Analysis Laboratory

基　　金：中国国家基础研究计划(2015CB856302,2015CB553602);国家自然科学基金(31070740,30930105,81101674);陕西省自然科学基金(2015JM3093,2014JQ4136);重点科技计划(2016SF-252)资助。

摘　　要：建立了测定橄榄叶、细胞培养基和血液样品中羟基酪醇(HT)含量的超高效液相色谱方法,并对影响分离分析的色谱条件进行优化。0.04%甲酸水溶液为流动相A,0.04%甲酸甲醇溶液为流动相B,采用线性梯度洗脱模式,流速0.2 mL/min,检测波长200 nm的最佳分离条件下,10 min内可以实现样品中低浓度HT(0.85μmol/L,S/N=10)的检测。该方法回收率的96.8%~111.5%,相关系数r^2=0.9997。An ultra-performance liquid chromatography method was established and optimized for determination of hydroxytyrosol(HT)in extractions of olive leaves,mediums and blood samples.Under the optimized conditions,setting 0.04%formic acid water and methanol solution as mobile phase A and B,respectively linear-gradient elution mode with a flow rate of 0.2 mL/min,low concentration of HT could be detected in 10 min with LOQ of 0.85μmol/L(S/N=10).The proposed method also performs good linearity range(r^2=0.9997)and satisfied recoveries(96.8%-111.5%),and shows great potential on routine assays of HT in real samples.

关 键 词：超高效液相色谱 羟基酪醇 橄榄叶 细胞培养基 血液 

分 类 号：O652.63[理学—分析化学]