鹅油甘油二酯与天蚕素抗菌肽合用对葡聚糖硫酸钠诱导的小鼠溃疡性结肠炎损伤的修复作用  

Repairing Effects of Goose Oil Diglyceride Combined with Cecropin Antimicrobial Peptide on Dextran Sodium Sulfate-Induced Ulcerative Colitis Injury in Mice

作　　者：徐慧心 王宝维[1,2] 葛文华 丛红霞 王茜 张名爱 孔敏[2] 凡文磊 XU Huixin;WANG Baowei;GE Wenhua;CONG Hongxia;WANG Qian;ZHANG Ming’ai;KONG Min;FAN Wenlei(Department of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China;National Waterfowl Industry Technical System Nutrition and Feed Function Laboratory,Qingdao 266109,China)

机构地区：[1]青岛农业大学食品科学与工程学院,青岛266109 [2]国家水禽产业技术体系营养与饲料功能研究室,青岛266109

出　　处：《动物营养学报》2020年第8期3877-3886,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：山东省2018年度农业重大应用技术创新项目;国家水禽产业技术体系专项基金(CARS-42-14);国家重点研发计划“绿色水禽高效安全养殖技术应用与示范”(2018YFD0501501)。

摘　　要：本试验旨在研究鹅油甘油二酯(GDG)与天蚕素抗菌肽(CAP)合用对葡聚糖硫酸钠(DSS)诱导的小鼠溃疡性结肠炎损伤的修复作用。试验分为2个阶段。造模阶段:将184只小鼠随机分为8个组,分别为对照组(Ⅰ组)、模型组(Ⅱ组)和修复组(Ⅲ~Ⅷ组)。对照组与模型组每组4个重复,每个重复8只;修复组每组4个重复,每个重复5只小鼠。对照组小鼠自由饮用蒸馏水,模型组和修复组小鼠均自由饮用5%DSS水溶液诱导小鼠肠道炎症,连续1周。修复阶段:将160只小鼠随机分成对照组(Ⅰ组)、模型组(Ⅱ组)和修复组(Ⅲ~Ⅷ组),每组4个重复,每个重复5只。对照组和模型组以生理盐水(100μL/只)灌胃;修复组给予不同水平的GDG和CAP灌胃,GDG添加水平分别为50、75、100μL/只,CAP添加水平分别为3.75、5.00 mL/kg BW。试验期为4周。结果表明:1)与Ⅰ组相比,Ⅱ组的造模后体重显著降低(P<0.05),造模后疾病活动指数(DAI)评分显著升高(P<0.05),结肠长度显著降低(P<0.05),血清白细胞介素-6和肿瘤坏死因子-α(TNF-α)含量显著升高(P<0.05),表明溃疡性结肠炎小鼠模型建立成功。2)Ⅲ~Ⅷ组修复后体重显著高于Ⅱ组(P<0.05),修复后DAI评分显著低于Ⅱ组(P<0.05)。3)Ⅲ~Ⅷ组的结肠长度显著高于Ⅱ组(P<0.05),结肠绒毛高度显著高于Ⅱ组(P<0.05),结肠隐窝深度显著低于Ⅱ组(P <0.05),结肠绒毛高度/隐窝深度显著高于Ⅱ组(P <0.05)。4)GDG与CAP合用对血清白细胞介素-4、白细胞介素-6、白细胞介素-17、白细胞介素-1β、TNF-α含量有显著交互作用(P<0.05)。由此可见,GDG和CAP合用可以有效治疗小鼠溃疡性结肠炎;小鼠按75μL/只GDG+5.00 mL/kg BW CAP灌胃对溃疡性结肠炎损伤的修复效果最佳。This experiment was conducted to study the effects of goose oil diglyceride(GDG)combined with cecropin antimicrobial peptide(CAP)on dextran sodium sulfate(DSS)-induced ulcerative colitis injury in mice.The experiment was divided into two phases.Modeling phase:a total of 184 mice were randomly divided into 8 groups,which were control group(groupⅠ),model group(groupⅡ)and repair groups(groupsⅢtoⅧ).Each control group and model group contained 4 replicates and 8 mice per replicate;each repair group contained 4 replicates and 5 mice per replicate.Mice in the control group were free to drink distilled water,and others in model group and repair groups were free to drink 5%DSS aqueous solution to induce the intestinal inflammation of mice,it lasted for 1 week.Repair phase:a total of 160 mice were randomly divided into control group(groupⅠ),model group(groupⅡ)and repair groups(groupsⅢtoⅧ),each group contained 4 replicates and 5 mice per replicate.Mice in the control group and model group were gavage with normal saline(100μL per mouse);mice repair groups were gavage with different levels of GDG and CAP,the GDG supplemental levels were 50,75,and 100μL per mouse,respectively;and the CAP supplemental levels were 3.75 and 5.00 mL/kg BW,respectively.The results showed as follows:1)compared with groupⅠ,the body weight after modeling of groupⅡwas significantly decreased(P<0.05),the disease activity index(DAI)score after modeling was significantly increased(P<0.05),the colonic length was significantly decreased(P<0.05),and the contents of interleukin-6 and tumor necrosis factor-α(TNF-α)in serum were significantly increased(P<0.05),it showed that the ulcerative colitis mice model successful build.2)The body weight after repairing of groupsⅢtoⅧwas significantly higher than that of groupⅡ(P<0.05),and the DAI score after repairing was significantly lower than that of groupⅡ(P<0.05).3)The colonic length of groupsⅢtoⅧwas significantly higher than that of groupⅡ(P<0.05),the colonic villus height was s

关 键 词：鹅油甘油二酯 天蚕素抗菌肽 小鼠 溃疡性结肠炎 修复 

分 类 号：S816[农业科学—饲料科学]