Regulation of Rice Tillering by RNA-Directed DNA Methylation at Miniature Inverted-Repeat Transposable Elements  

作　　者：Le Xu Kun Yuan Meng Yuan Xiangbing Meng Min Chen Jianguo Wu Jiayang Li Yijun Qi 

机构地区：[1]Center for Plant Biology,School of Life Sciences,Tsinghua University,Beijing 100084,China [2]Tsinghua-Peking Center for Life Sciences,Beijing 100084,China [3]Key Laboratory of Plant Genomics and National Center for Plant Gene Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China [4]Vector-borne Virus Research Center,State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Province Key Laboratory of Plant Virology,Institute of Plant Virology,Fujian Agriculture and Forestry University,Fuzhou 350002,China

出　　处：《Molecular Plant》2020年第6期851-863,共13页分子植物（英文版）

基　　金：This work was supported by grants from National Natural Science Foundation of China(grant no.31788103);to J.L.and Y.Q.and the National Key R&D Program of China(grant no.2016YFA0500800);to Y.Q.Y.Q.is a visiting investigator of the CAS Center for Excellence in Molecular Plant Sciences.

摘　　要：Tillering is a major determinant of rice plant architecture and grain yield.Here,we report that depletion of rice OsNRPD1a and OsNRPD1b,two orthologs of the largest subunit of RNA polymerase IV,leads to a high-tillering phenotype,in addition to dwarfism and smaller panicles.OsNRPD1a and OsNRPD1b are required for the production of 24-nt small interfering RNAs that direct DNA methylation at transposable elements(TEs)including miniature inverted-repeat TEs(MITEs).Interestingly,many genes are regulated either positively or negatively by TE methylation.Among them,OsMIR156d and OsMIR156j,which promote rice tillering,are repressed by CHH methylation at two MITEs in the promoters.By contrast,D14,which suppresses rice tillering,is activated by CHH methylation at an MITE in its downstream.Our findings reveal regulation of rice tillering by RNA-directed DNA methylation at MITEs and provide potential targets for agronomic trait enhancement through epigenome editing.

关 键 词：RDDM siRNA MITES OsMIR156 D14 TILLER 

分 类 号：S511[农业科学—作物学]