持绿大豆叶片中叶绿素降解途径关键酶基因的表达分析  

Expression Analysis of Key Enzyme Gene in Chlorophyll Degradation Pathway in Stay Green Soybean Leaves

作　　者：张鑫 莫翱伟 许鹏昊 张卿[1] 王程[1] 谢皓[1] Zhang Xin;Mo Aowei;Xu Penghao;Zhang Qing;Wang Cheng;Xie Hao(Key Laboratory of New Technology in Agricultural Application,College of Plant Science and Technology,Beijing University of Agriculture,Beijing,102206)

机构地区：[1]北京农学院植物科学技术学院,北京农业应用新技术北京市重点实验室,北京102206

出　　处：《分子植物育种》2020年第15期4871-4877,共7页Molecular Plant Breeding

基　　金：国家自然科学基金(31371648);北京市教育委员会科研项目一般计划(KM201810020006);北京农学院教师科研基金项目(SXQN2016104)共同资助。

摘　　要：为了探究叶绿素降解途径关键酶基因在大豆不同生育时期的表达情况,以期为揭示调控大豆持绿特性的分子机制提供参考,本研究以大豆‘科丰14’和其持绿突变体‘北农108’为试验材料,在其苗期、开花期和成熟前期,利用荧光定量qPCR方法分析了6种叶绿素降解途径中关键酶基因表达量的变化。结果表明,6种关键酶基因在两个品种苗期和开花期的相对表达量并无显著差异,在成熟前期脱镁螯合酶(MDCase)、脱镁叶绿素酶(PPH)和脱镁叶绿酸a加氧酶(PAO)三个酶基因相对表达量显著提高(p<0.05),并且在‘科丰14’中的表达量远高于在‘北农108’中的表达量,因此推测在叶绿素降解代谢途径中这三个关键酶基因所调控的代谢过程可能是‘北农108’持绿的重要原因之一。本研究为探究大豆持绿特性的分子调控机制,作用机理提供实践参考,为高光效大豆的遗传改良,探究植物衰老延缓机制,提高产量和品质等提供理论支持。In order to explore the expression of key enzyme genes of chlorophyll degradation pathway in different growth stages of soybean,and to provide a reference for revealing the molecular mechanism regulating the green retention of soybean.We used soybean ’Kefeng14’ and its green mutant ’Beinong108’ as experimental materials.The quantitative qPCR method was used to analyze the changes of the expression levels of key enzyme genes in the six chlorophyll degradation pathways at seedling stage,flowering stage and pre-mature stage.The results showed that the relative expression levels of the six enzyme genes in the two varieties were not significantly different at the seedling stage and the flowering stage.In the early stage of maturation,the relative expression of the three enzyme genes,demagnesium chelatase(MDCase),pheophytinase(PPH) and pheophorbide(PAO),was significantly increased(p<0.05),and the expression level in ’Kefeng14’ was much higher than that in ’Beinong108’,so it was speculated that the chlorophyll degradation pathway of metabolic processes regulated by these three key enzyme genes are one of the important reasons for ’Beinong108’ to stay green.The research might provide a practical reference for exploring the molecular regulation mechanism of stay green gene and provide theoretical support for the genetic improvement of high light efficiency soybean,exploring the mechanism of plant aging retardation,improving yield and quality.

关 键 词：大豆 叶绿素 酶基因 荧光定量PCR 

分 类 号：S565.1[农业科学—作物学]