Evaluation of drought tolerance in ZmVPP1-overexpressing transgenic inbred maize lines and their hybrids  

作　　者：JIA Teng-jiao LI Jing-jing WANG Li-feng CAO Yan-yong MA Juan WANG Hao ZHANG Deng-feng LI Hui-yong 

机构地区：[1]Institute of Cereal Crop,Henan Academy of Agricultural Sciences,Zhengzhou 450002,P.R.China [2]Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100089,P.R.China

出　　处：《Journal of Integrative Agriculture》2020年第9期2177-2187,共11页农业科学学报（英文版）

基　　金：This study was supported by the National Key Project for Research on Transgenic Plants,China(2016Zx08003-O04);the Independent Innovation Project of Henan Academy of Agricultural Sciences,China(2060302).

摘　　要：The vacuolar proton-pumping pyrophosphatase gene(VPP)is often used to enhance plant drought tolerance via genetic engineering.In this study,the drought tolerance of four transgenic inbred maize lines overexpressing ZmVPP1(PH4CV-T,PH6WC-T,Chang7-2-T,and Zheng58-T)and their transgenic hybrids was evaluated at various stages.Under normal and drought conditions,the height and fresh weight were greater for the four transgenic inbred maize lines than for the wild-type(WT)controls at the germination and seedling stages.Additionally,the transgenic plants exhibited enhanced photosynthetic efficiency at the seedling stage.In irrigated and non-irrigated fields,the four transgenic lines grew normally,but with increased ear weight and yield compared with the WT plants.Moreover,the ear weight and yield of the transgenic hybrids resulting from the PH4CV-T×PH6WC-W and Chang7-2-T×Zheng58-W crosses increased in the non-irrigated field.Our results demonstrated that the growth and drought tolerance of four transgenic inbred maize lines with improved photosynthesis were enhanced by the overexpression of ZmVPP1.Moreover,the Chang7-2 and PH4CV transgenic lines may be useful for future genetic improvements of maize hybrids to increase drought tolerance.

关 键 词：inbred maize lines drought stress ZmVPP1 PHOTOSYNTHESIS 

分 类 号：S513[农业科学—作物学]