ED-XRF法快速测定虾预混合饲料中钙、磷含量  

Rapid Determination of Calcium and Phosphorus Contents in Shrimp Premix Feed by ED-XRF Method

作　　者：刘召贵[1] 张辉[1] 赵鹏[1] 吴敏[1] 李强[1] LIU Zhao-gui;ZHANG Hui;ZHAO Peng;WU Min;LI Qiang(Jiangsu Skyray Instrument Co.,Ltd.,Kunshan215300,China)

机构地区：[1]江苏天瑞仪器股份有限公司,江苏昆山215300

出　　处：《畜牧与饲料科学》2020年第4期1-7,共7页Animal Husbandry and Feed Science

摘　　要：虾预混合饲料中钙(Ca)、磷(P)含量直接影响虾的生长、发育和健康状况。传统的化学分析方法需要对虾预混合饲料进行复杂的预处理,取得测试结果需要花费数小时。采用一种简单、快捷的分析方法——能量色散X射线荧光光谱法(ED-XRF),对虾预混合饲料进行粉碎、压片和测试,在数分钟内获得了样品的测试结果。ED-XRF法对Ca、P的检出限分别为43 mg/kg和163 mg/kg,定量限分别为142 mg/kg和544 mg/kg。对虾预混合饲料3#样本连续测试6次,Ca、P的连续测定数据的极差分别为0.159%和0.013%,分别小于推荐性国家标准中Ca、P含量检测的重复性临界差CrR95(6)0.239%和0.031%,表明ED-XRF法满足推荐性国家标准对饲料中Ca、P含量测定的要求。通过对10个已定值的虾预混合饲料样品进行Ca、P含量测定,ED-XRF法的测定值与推荐性国家标准中相关方法测定值的相对差值不大于4.55%。综上表明,ED-XRF法具有较高的精密度和准确度,可用于虾预混合饲料生产企业对Ca、P元素添加量的监控。The contents of calcium(Ca) and phosphorus(P) in premix feed directly affected the growth, development and health status of shrimp. Traditional chemical analysis methods required complicated pre-treatment process for shrimp premix feed and always took several hours to obtain test results. In this study, a simple and fast analysis method, energy dispersive X-ray fluorescence spectrometry(ED-XRF), was used to assess the contents of Ca and P in shrimp premix feed, and the test results of the samples were obtained within a few minutes. The pre-treatment process of ED-XRF method only included comminution and squash. The limit of detection of ED-XRF method for Ca and P were 43 mg/kg and 163 mg/kg, respectively, and the limit of quantification of that for Ca and P were 142 mg/kg and 544 mg/kg, respectively. Six consecutive determinations of shrimp premix feed sample 3# were conducted by ED-XRF method. The range of the continuous measurement data of Ca and P were 0.159% and 0.013%, respectively, which were smaller than the repeatability critical difference of CrR95(6) for the contents detection of Ca(0.239%) and P(0.031%) in the recommended national standard, indicating that the ED-XRF method met the requirements of the recommended national standard for the determination of Ca and P contents in feed. By analyzing the contents of Ca and P of ten shrimp premix feed samples which were measured by national standard methods, we found that the relative differences in measured value of Ca and P contents between ED-XRF method and recommended national standard method did not exceeded4.55%. To sum up, the ED-XRF method had high precision and accuracy, and it could be used in monitoring the addition of Ca and P for shrimp premix feed production enterprises.

关 键 词：能量色散X射线荧光光谱法 虾预混合饲料 钙含量 磷含量 快速检测 

分 类 号：S816.17[农业科学—饲料科学]