微囊化益生菌对小鼠急性溃疡性结肠炎的影响  

Effects of Microencapsulated Probiotics on Ulcerative Colitis in Mice

作　　者：张琳[1,2] 綦文涛 刘延国 ZHANG Lin;QI Wentao;LIU Yanguo(Hebei Key Laboratory of Animal Diversity, Langfang Normal University,Langfang 065000,China;Academy of National Food and Strategic Reserves Administration,Beijing 100037,China;Beijing Abest Sci & Tech Development Co.,Ltd.,Beijing 102600,China)

机构地区：[1]廊坊师范学院,河北省动物多样性重点实验室,廊坊065000 [2]国家粮食和物资储备局科学研究院,北京100037 [3]北京安佰特科技发展有限公司,北京102600

出　　处：《中国畜牧兽医》2020年第8期2571-2578,共8页China Animal Husbandry & Veterinary Medicine

基　　金：河北省自然科学基金项目(C2020408003);廊坊市科学技术研究与发展计划自筹经费项目(2020013020)。

摘　　要：试验旨在观察微囊化布拉迪酵母菌(Saccharomyces boulardii,S.boulardii)与微囊化粪肠球菌(Enterococcus faecium,E.faecalis)对小鼠溃疡性结肠炎(UC)的治疗效果并探讨其作用机制。采用3%葡聚糖硫酸钠(dextran sulfacte sodium,DSS)灌胃法制备小鼠UC动物模型,试验共分为6组,其中饮用DSS的小鼠随机分为5组:粪肠球菌菌粉组、微囊化粪肠球菌组、布拉迪酵母菌菌粉组和微囊化布拉迪酵母菌组给予不同的药物治疗,药物皆以溶液形式灌胃给药,UC模型小鼠(DSS组)每天饮用与药物等体积的生理盐水;正常对照组灌服与药物等体积的生理盐水。观察UC小鼠的症状和组织学变化,ELSIA检测血清中细胞因子(IL-6、IL-10与TNF-α)的含量,Western blotting检测小鼠结肠黏膜紧密连接蛋白(Occludin和Claudin-1)表达量。结果显示,与模型组相比,治疗后各益生菌组小鼠血清中TNF-α和IL-6含量均显著降低(P<0.05),IL-10含量显著升高(P<0.05),微囊化益生菌组小鼠髓过氧化物酶(MPO)活性及结肠损伤组织学评分(粪肠球菌粉组除外)均显著下降(P<0.05),布拉迪酵母菌菌粉组与微囊化布拉迪酵母菌小鼠结肠黏膜内Occludin和Claudin-1表达量均显著升高(P<0.05)。粪肠球菌和布拉迪酵母菌可以缓解UC模型小鼠结肠炎症病变,且经过微囊化后的粪肠球菌和布拉迪酵母菌表现出更好的缓解小鼠急性溃疡性结肠炎病症效果,其机制与粪肠球菌和布拉迪酵母菌可以降低血清中IL-6与TNF-α含量,提高Occludin与Claudin-1的表达量密切相关。This study was aimed to observe the effects of microencapsulated Saccharomyces boulardii(S.boulardii)and Enterococcus faecium(E.faecalis)on ulcerative colitis(UC)and discuss its possible mechanism in mice.Mice model with UC was established by 3%dextran sulphate sodium(DSS),the test was divided into 6 groups,the model mice were randomized into 5 groups,mice were treated with unencapsulated-free E.faecalis,microencapsulated E.faecalis,unencapsulated-free S.boulardii and microencapsulated S.boulardii solution,respectively,and saline solution in DSS group by oral gavage.UC model mice(DSS group)were fed with the same volume of saline as the drug every day,while the normal control group was fed with the same volume of saline solution.The symptoms and histological changes of UC mice were observed,the pro-inflammatory cytokines including IL-6,IL-10 and TNF-αwere measured by ELISA,and the expression of the Occludin and Claudin-1 were measured by Western blotting.The results showed that compared with DSS group,the contents of TNF-αand IL-6 were significantly reduced in probiotics groups(P<0.05),and the content of IL-10 was significantly increased(P<0.05).The myeloperoxidase activities and histoligical scores(except for unencapsulated-free E.faecalis group)were significantly decreased in microencapsulated pribiotics groups after treatment(P<0.05).The expression of Occludin and Claudin-1 were significantly increased inunencapsulated-free S.boulardii and microencapsulated S.boulardii groups(P<0.05).E.faecalis and S.boulardii could reduce the inflammatory lesions in the colon of UC mice,and the microencapsulated pribiotics had a better effect,moreover,the mechanisms of the improvement were possibly related to the decrease of the contents of IL-6 and TNF-α,and the increase of the expression of Occludin and Claudin-1.

关 键 词：乳化凝胶化 微囊化益生菌 布拉迪酵母菌 粪肠球菌 结肠炎 

分 类 号：Q939.121[生物学—微生物学]