康宁木霉对龟裂链霉菌MY02诱导活性及其诱导组分的初步分析  

Inducing Activity and Preliminary Analysis for Inducing Components of Trichoderma koningii to Streptomyces rimosus MY02

作　　者：魏溪垚 刘秋 于基成 WEI Xi-yao;LIU Qiu;YU Ji-cheng(Coll,of Life Sci.,Dalian Minzu Uni.,Dalian 116600)

机构地区：[1]大连民族大学生命科学学院,辽宁大连116600

出　　处：《微生物学杂志》2020年第3期28-32,共5页Journal of Microbiology

基　　金：国家自然科学基金项目(31770006);中央高校基本科研业务费专项资金资助项目(wd01187,wd01189);大学生创新创业训练项目(201812026217,201812026205)。

摘　　要：利用真菌作为诱导菌株评价其对龟裂链霉菌(Streptomyces rimosus)MY02的活性代谢产物的影响,并对其诱导条件进行优化。当以康宁木霉(Trichoderma koningii)和香菇真菌(Lentinus edodes)作为诱导菌株,黄瓜枯萎病菌(Fusarium oxysporum f sp.cucumarinum)作为指示菌,以菌株MY02作为被诱导菌株时,康宁木霉和香菇真菌对菌株MY02的抗真菌活性均具有明显的正向诱导作用,菌株MY02的抗真菌活性均比对照明显增强。以康宁木霉作为诱导菌株时,其最佳诱导条件为接种量2%,添加时间为发酵36 h。通过初步分析康宁木霉的诱导组分为其发酵产生的多糖类物质。The effect of antifungal active metabolite of Streptomyces rimosus MY02 was evaluated by using fungi as inducers,meanwhile,its induction conditions were optimized.When Trichoderma koningii and Lentinus edodes were used as inducer strains,and Fusarium oxysporum f sp.cucumarinum as an indicator,and when take strain MY02 as induced strain,T.koningii and L.edodes possessed positive induction effect on antifungal activities of strain MY02,and the antifungal activity of MY02 increased significantly as compared with control.When take T.koningii as inducer strain,its optimum induction condition was at 2%of inoculation volume,with the adding time at 36 h.Initial analysis showed that the inducing components of T.koningii was its polysaccharides of fermented production.

关 键 词：真菌诱导子 康宁木霉 龟裂链霉菌 次级代谢产物 诱导活性 

分 类 号：Q939.5[生物学—微生物学]