基于ICP-MS的拟穴青蟹蜕壳期前后蟹肉Ca^2+、Mg^2+浓度变化研究  

Study on Ca^2+,Mg^2+ concentration in crab meat before and after molting stage of Scylla paramamosain by ICP-MS

作　　者：周冬仁[1] 林锋[1] 孙博弈 盛鹏程[1] 高晟 郝贵杰[1] 叶雪平[1] ZHOU Dong-ren;LIN Feng;SUN Bo-yi;SHENG Peng-cheng;GAO Sheng;HAO Gui-jie;YE Xue-ping(Ministry of Agriculture and Rural Affairs Key Laboratory of Healthy Freshwater Aquaculture/Key Laboratory of Fish Health and Nutrition of Zhejiang Province/Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,Zhejiang,China)

机构地区：[1]农业农村部淡水渔业健康养殖重点实验室/浙江省鱼类健康与营养重点实验室/浙江省淡水水产研究所,浙江湖州313001

出　　处：《湖北农业科学》2020年第12期114-116,共3页Hubei Agricultural Sciences

基　　金：浙江省科技厅重点研发项目(2018C02019)。

摘　　要：采用石墨微波消解技术处理拟穴青蟹(Scylla paramamosain)蟹肉样品,ICP-MS同时测定Ca、Mg两种元素,标准曲线相关系数均≥0.995,前处理耗时短,消解效果良好。结果表明,拟穴青蟹在硬壳期时体内Ca^2+、Mg^2+平均含量为471、2508 mg/kg,而蜕壳后分别为385、1565 mg/kg,拟穴青蟹蜕壳前后Mg^2+浓度差异不显著、Ca^2+浓度差异显著。因此,为使拟穴青蟹生长过程中甲壳顺利蜕壳及硬化,建议在养殖池中合理补充Ca^2+、Mg^2+。The meat samples of Scylla paramamosain were treated by graphite microwave digestion.ICP-MS was used to determine Ca^2+ and Mg^2+ simultaneously.The correlation coefficient of standard curve was ≥0.995.The pretreatment time was short and the diges⁃tion effect was good.The results showed that the average contents of Ca^2+ and Mg^2+ in S.paramamosain were 471 and 2508 mg/kg in hard shell stage,385 and 1565 mg/kg in molting stage respectively.there had no significant differences in Mg^2+,and had significant dif⁃ference in Ca^2+ concentration before and after molting stage.In order to make the Scylla molt and harden smoothly during the growth of the crab,it was suggested that Ca^2+,Mg^2+ should be supplemented reasonably in the culture pond.

关 键 词：拟穴青蟹(Scylla paramamosain) CA^2+ MG^2+ 电感耦合等离子体-质谱 

分 类 号：S968.251[农业科学—水产养殖]