白桦BpPR1基因生物信息学分析  

Bioinformatics analysis of BpPR1 gene from Betula platyphylla

作　　者：安玉婷 李然红[1] 陈鑫[1] 刘丹[1] AN Yu-ting;LI Ran-hong;CHEN Xin;LIU Dan(Mudanjiang Normal University,Mudanjiang 157012,Heilongjiang,China)

机构地区：[1]牡丹江师范学院,黑龙江牡丹江157012

出　　处：《湖北农业科学》2020年第12期171-174,178,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31800558)。

摘　　要：利用生物信息学方法,对白桦BpPR1理化性质、亲水/疏水性、跨膜结构、二级结构、三级结构及与其他植物PR1蛋白的同源性进行了预测,同时对该基因启动子的顺式作用元件以及基因结构进行了预测和分析。结果表明,①BpPR1的cDNA全长为816 bp,CDS为531 bp,编码由176个氨基酸构成的稳定亲水性蛋白,存在跨膜运输结构,属于CAP蛋白家族的一员。白桦BpPR1与葡萄、枣亲缘关系较近;②BpPR1基因组结构中含有2个外显子和3个内含子;③BpPR1启动子含有多个真核生物启动子的基本元件CAAT-box和TATA-box,还含有与低温响应、脱落酸响应、茉莉酸甲酯响应、生长素响应、水杨酸响应、细胞分裂素响应相关的响应元件以及MYBHv1结合位点等,说明该蛋白可能受多种生长调节剂调控,参与生物与非生物胁迫过程。Using bioinformatics methods,physical and chemical properties of white birch BpPR1,hydrophilic/hydrophobic,across the membrane structure,secondary structure and tertiary structure and with some other plants PR1 protein homology were forecasted,the cis function of the gene promoter elements and gene structure prediction and analysis were performed.The results showed that:①the total length of cDNA of BpPR1 was 816 bp,and the CDS was 531 bp.The encoding protein was a hydrophilic stable protein com⁃posed of 176 amino acids.It had a transmembrane transport structure and belonged to the CAP protein family.Birch BpPR1 was close⁃ly related to grape and jujube.②its genome structure contained 2 exons and 3 introns.③BpPR1 promoter contained multiple eukaryot⁃ic promoter CAAT box and the basic components-TATA box,also contained low temperature response,the response of abscisic acid and methyl jasmonic acid,auxin response and the response of salicylic acid and cytokinin response related components,as well as MYBHv1 binding sites and so on,indicating that the protein may be a variety of hormones control,to participate in the process of bio⁃logical and abiotic stress.

关 键 词：白桦(Betula platyphylla) 病程相关蛋白 生物信息学 

分 类 号：Q789[生物学—分子生物学]