西瓜品种‘黑皮’对瓜蚜的抗性遗传分析  

Genetic Analysis of Resistance of Watermelon Cultivars‘Heipi’to Aphis gossypii

作　　者：陈青[1,2,3,4] 梁晓 伍春玲[1,2,3,4] 刘迎 徐雪莲[1,2,3,4] CHEN Qing;LIANG Xiao;WU Chunling;LIU Ying;XU Xuelian(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;Key Laboratory of Pests Comprehensive Governance for Tropical Crops,Ministry of Agriculture and Rural Affairs,Haikou,Hainan 571101,China;Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests,Haikou,Hainan 571101,China;Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests,Haikou,Hainan 571101,China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南海口571101 [2]农业农村部热带作物有害生物综合治理重点实验室,海南海口571101 [3]海南省热带作物病虫害生物防治工程技术研究中心,海南海口571101 [4]海南省热带农业有害生物监测与控制重点实验室,海南海口571101

出　　处：《热带作物学报》2020年第7期1415-1419,共5页Chinese Journal of Tropical Crops

基　　金：农业农村部财政专项“南峰专项二期”(No.NFZX2018);海南省重点研发计划项目(No.ZDYF2018238);中央级公益性科研院所基本科研业务费专项(No.1630042019025)。

摘　　要：采用单株蚜量比值法室内鉴定与RAPD、SCAR标记相结合的方法,系统开展了西瓜品种‘黑皮’对瓜蚜的抗性遗传分析。结果表明,以‘黑皮’和‘花绿’为亲本的杂交F1代对瓜蚜表现为抗性,自交F2代植株表型出现抗感性分离,抗蚜与感蚜植株分离比经χ2测验符合3∶1分离规律,并用筛选获得的RAPD标记WO4600和SCAR标记WO4-S530验证了4个抗蚜品种、4个感蚜品种和10个F2抗蚜单株、10个F2感蚜植株。结果以‘黑皮’为亲本的西瓜抗蚜性由单显性核基因控制且能稳定遗传,‘黑皮’‘绿美人’‘黑美人’和‘惠兰’品种对瓜蚜具有良好抗性,‘花绿’‘凤光’‘蕙宝’和‘甜美人’品种则易感蚜虫。To get stable resistant watermelon cultivars,the genetic analysis of the resistance of watermelon cultivar‘Heipi’to Aphis gossypii was proceeded by the ratio of aphid number at seedling in a greenhouse added with RAPD and SCAR.The reciprocal-hybrid F1 of‘Heipi’and‘Hualv’was resistant while the resistant and susceptible plants in F2 hybrid fitted the separation ratio of 3:1 byχ2 test.Four resistant cultivars,four susceptible cultivars,ten resistant F2 single plants and ten susceptible F2 single plants were verified with screened RAPD WO4600 and SCAR WO4-S530.The results indicated that the resistance of the parent‘Heipi’to A.gossypii was controlled by a dominant single gene which was stably inherited,‘Heipi’,‘Lvmeiren’,‘Heimeiren’and‘Huilan’were aphid-resistant cultivars,‘Hualv’,‘Fengguang’,‘Huibao’and‘Tianmeiren’were aphid-susceptible cultivars.

关 键 词：西瓜品种 瓜蚜 抗蚜性 遗传分析 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]