宁夏部分地区奶牛犊牛隐孢子虫感染情况调查  

Investigation of Cryptosporidium infection in dairy calves in partial areas of Ningxia

作　　者：高海慧 施安 张立强 厉龙 王培柱 吴学青 康晓冬 GAO Hai-hui;SHI An;ZHANG Li-qiang;LI Long;WANG Pei-zhu;WU Xue-qing;KANG Xiao-dong(Institute of Animal Science,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,750002,China;Ningxia Agricultural Reclamation Helanshan Dairy Co.Ltd,Yinchuan 720002,China;Animal Disease Prevention and Control Center of Huinong District,Shizuishan City,Pingluo,Ningxia 753200,China;Animal Husbandry and Veterinary Station,Biandangou Town,Litong District,Wuzhong,Ningxia 751100,China)

机构地区：[1]宁夏农林科学院动物科学研究所,宁夏银川750002 [2]宁夏农垦贺兰山奶业有限公司,宁夏银川720002 [3]宁夏石嘴山市惠农区动物疾病预防控制中心,宁夏平罗753200 [4]宁夏吴忠市利通区扁担沟镇畜牧兽医工作站,宁夏吴忠751100

出　　处：《中国兽医学报》2020年第7期1347-1350,共4页Chinese Journal of Veterinary Science

基　　金：宁夏农林科学院农业科技自主创新基金资助项目(NKYQ-18-13,NKYQ-19-08);宁夏回族自治区自然科学基金资助项目(2018AAC03185,NZ16102)。

摘　　要：为了解宁夏地区犊牛隐孢子虫的感染情况,从3个市8个规模化奶牛场采集272份犊牛粪便样品,利用巢氏PCR技术对18S rRNA基因位点进行检测和分析。结果显示,隐孢子虫的总感染率为12.88%,银川市隐孢子虫感染率为16.89%,吴忠市隐孢子虫感染率为6.94%,石嘴山市隐孢子虫感染率为9.62%,三者差异不显著(P>0.05),断奶前、后感染率分别为14.14%,12.14%。序列处理分析发现3种隐孢子虫,分别为牛隐孢子虫(Cryptosporidium bovis,C.bovis)、瑞氏隐孢子虫(Cryptosporidium ryanae,C.ryanae)和微小隐孢子虫(Cryptosporidium parvum,C.parvum),其中C.bovis分离率最高(54.29%)。研究表明这些地区犊牛隐孢子虫感染比较普遍,且感染的虫种有3种,其为深入了解宁夏部分地区隐孢子虫的分子流行情况提供了参考数据。To understand the prevalence of Cryptosporidium in dairy calves in partial regions of Ningxia.272 fecal samples were collected from eight cattle farms in three cities.All fecal samples were examined and analyzed by the PCR based on 18 S rRNA genes locus.The results showed that the total infection rate was 12.88%,of which 16.89%from Yinchuan city,9.62%from Shizuishan city,and 6.94%from Wuzhong city,no significant differences were observed among different farms(P>0.05).pre-weaned and post-weaned infection rate was 14.14%and 12.14%.Three species were found,Cryptosporidium bovis,Cryptosporidium ryanae and Cryptosporidium parvum,Cryptosporidium bovis was the most prevalence species(54.29%).These finding suggested that Cryptosporidium infection in dairy calves is common,The results supply an epidemiological data for Cryptosporidium molecular prevalence in partial areas of Ningxia.

关 键 词：宁夏 犊牛 隐孢子虫 

分 类 号：S852.7[农业科学—基础兽医学]