生物炭基Rs-198菌剂对辣椒的耐盐促生性能研究  

Effects of biochar inoculated Pseudomonas putida Rs-198 on salt tolerance of pepper

作　　者：魏萌萌 刘啸尘 何艳慧[1] 武占省[1,2] WEI Mengmeng;LIU Xiaochen;HE Yanhui;WU Zhansheng(School of Chemistry and Chemical Engineering,Shihezi University,Shihezi,Xinjiang 832003,China;School of Environmental and Chemical Engineering,Xi′an Polytechnic University,Xi′an,Shanxi 710048,China)

机构地区：[1]石河子大学化学化工学院,新疆石河子832003 [2]西安工程大学环境与化学工程学院,陕西西安710048

出　　处：《石河子大学学报（自然科学版）》2020年第4期428-433,共6页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(U1803332)。

摘　　要：针对土壤盐渍化问题日益严重,创新性的将生物炭与植物根际促生菌(PGPR,Plant growth-promoting rhizobacteria)有效结合,以更好地实现提高作物耐盐碱性和促进作物生长的目的。本研究将生物炭基Rs-198菌剂(BBRs,Biochar inoculated Pseudomonas putida Rs-198)应用到盐胁迫条件下生长的辣椒植物中,探究BBRs菌剂在盐胁迫下的功效和缓解盐胁迫的机理。结果表明,在0.5%NaCl胁迫下,与空白对照CK2相比,BBRs2处理的辣椒株高、根长、鲜重和干重分别增加了26.43%、24.27%、42.59%和50.00%。茎粗、叶面积、叶片数和相对水分含量分别提高了23.41%、14.03%、12.79%和3.54%。叶绿素a、b分别增加了47.22%和62.50%。可溶性蛋白、可溶糖、脯氨酸和电解质渗透率分别降低了33.07%、57.78%、62.20%和36.88%。SOD、PPO和POD分别降低了35.68%、31.85%和37.20%。表明BBRs通过降低渗透物质和抗氧化酶的积累缓解了盐胁迫,促进了辣椒生长,为解决盐胁迫问题提供了思路和方法,有利于农业可持续发展。In view of the increasingly serious problem of soil salinization,biochar and plant growth-promoting rhizobacteria(PGPR)are innovatively combined to improve the salt and alkali tolerance of crops and promote the growth of crops.In this study,biochar inoculated Pseudomonas putida Rs-198(BBRs)was applied to pepper plants under salt stress to investigate the efficacy and the alleviating salt stress mechanism of BBRs.The results showed that under 0.5%NaCl stress,compared with blank control CK2,after applying BBRs2,the plant height,root length,fresh weight and dry weight of pepper increased by 26.43%,24.27%,42.59%and 50.00%,respectively.Stem diameter,leaf area,leaf number and relative water content increased by 23.41%,14.03%,12.79%and 3.54%,respectively.Chlorophyll a,chlorophyll b increased by 47.22%,62.50%,respectively.Soluble protein,soluble sugar,proline and electrolyte leakage decreased by 33.07%,57.78%,62.20%and 36.88%,respectively.SOD,PPO and POD were decreased by 35.68%,31.85%and 37.20%respectively.The results showed that BBRs alleviated salt stress and promoted the growth of pepper by reducing the accumulation of osmotic substances and antioxidant enzymes,which provided ideas and methods for solving the problem of salt stress and was conducive to the sustainable development of agriculture.

关 键 词：盐胁迫 生物炭基Rs-198菌剂 辣椒 

分 类 号：S156[农业科学—土壤学]