双波长分光光度法快速测定母乳替代品中钙磷比  

Rapid Determination of Calcium/Phosphorus Ratio in Breast Milk Substitutes by Dual Wavelength Spectrophotometry

作　　者：王军锋 李姝颖 朱凝 李敏 陈思轩 黄滢 WANG Jun-feng;LI Shu-ying;ZHU Ning;LI Min;CHEN Si-xuan;HUANG Ying(School of Chemistry&Chemical Engineering,Shaanxi Xueqian Normal University,Xian 710100,China)

机构地区：[1]陕西学前师范学院化学化工学院,陕西西安7100100

出　　处：《食品与药品》2020年第4期268-271,共4页Food and Drug

基　　金：国家级、陕西省级和陕西学前师范学院大学生创新创业训练计划项目资助(2018DC065,201814390007,201841007)。

摘　　要：目的建立双波长分光光度法,不经分离同时测定母乳替代品中钙、磷含量。方法常温下,pH 5.4醋酸-醋酸钠缓冲溶液中,先加入磷系列显色溶液使其反应30 min,后加入钙显色剂反应10 min,710 nm测定吸光度实现磷的测定,556 nm处利用差值吸光度完成对钙的测定。结果在上述实验条件下,磷和钙的浓度分别在0.3~1.7μg/ml,0.4~2.0μg/ml范围内与吸光度呈良好的线性关系,样品加标回收率在96.7%~112.3%之间。结论此法准确度高,方便快捷,所用仪器简单,用于母乳替代品中钙磷比测定,结果满意。Objective To establish a dual-wavelength spectrophotometry for the simultaneous determination of calcium and phosphorus in breast milk substitutes without separation. Methods At 25 ℃ and in acetic acid-sodium acetate buffer solution(pH 5.4), the phosphorus series color developing solution was added to react for 30 min, then the calcium color developing agent was added to react for another 10 min. The absorbance was measured at 710 nm to realize the determination of phosphorus, and the differential absorbance at 556 nm was used to complete the measurement of calcium. Results There were good linear relationships between absorbance and concentration for phosphorus of 0.3-1.7 μg/ml and for calcium of 0.4-2.0 μg/ml, respectively. The recovery was between 96.7 % and 112.3 %. Conclusion This method is highly accurate, convenient and fast, and the instrument used is simple, thus can be used to determine Ca/P ratio in breast milk substitutes with satisfactory results.

关 键 词：分光光度法 钙磷比 同时测定 

分 类 号：TS252.51[轻工技术与工程—农产品加工及贮藏工程]