解淀粉芽孢杆菌SJ06脂肽粗提物与吡唑醚菌酯协同对玉米小斑病菌的抑制效果  

Synergistic inhibitory activity of crude lipopeptide extracts from Bacillus amyloliquefaciens strain SJ06 and pyraclostrobin against Bipolaris maydis

作　　者：段海明 李可文 DUAN Haiming;LI Kewen(College of Agriculture,Anhui Science and Technology University,Fengyang,Anhui 233100,China)

机构地区：[1]安徽科技学院农学院,安徽凤阳233100

出　　处：《湖南农业大学学报（自然科学版）》2020年第4期432-436,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：安徽省教育厅自然科学研究项目(KJ2020A0067);教育部大学生创新创业训练计划项目(201810879031)。

摘　　要：运用对峙培养法测定解淀粉芽孢杆菌SJ06菌株对玉米小斑病菌的拮抗活性;采用菌丝生长速率法测定发酵上清液、脂肽粗提物对玉米小斑病菌的抑菌活性,以及SJ06脂肽粗提物与吡唑醚菌酯复配的协同抑菌活性。结果表明:对峙培养SJ06对玉米小斑病菌的抑制率为71.43%;SJ06发酵上清液和脂肽粗提物对病菌的EC50值分别为0.88μL/mL和0.23μL/mL,吡唑醚菌酯对病菌的EC50值为1.10μg/m L;SJ06脂肽粗提物与吡唑醚菌酯复配对玉米小斑病菌的毒性比均大于1,说明复配对病菌的抑制效果均为增效作用,其中,复配体积比为4∶6时毒性比为1.21,增效作用最强。The antagonistic activity of Bacillus amyloliquefaciens strain SJ06 against Bipolaris maydis was tested by confrontation culture, and the antifungal activity of fermentation supernatant, crude lipopeptide extracts and pyraclostrobin were determined by mycelial growth rate. The synergic antifungal activity of crude lipopeptide extracts of strain SJ06 and pyraclostrobin was obtained by mixing them in different proportion. The results showed that the inhibition rate of strain SJ06 against Bipolaris maydis was 71.43%. The EC50 of fermentation supernatant and crude lipopeptide extracts to Bipolaris maydis was 0.88 μL/mLand 0.23 μL/mL, respectively. The EC50 value of pyraclostrobin to Bipolaris maydis was 1.10 μg/mL. The toxicity ratio of the combination of crude lipopeptide extracts and pyraclostrobin to Bipolaris maydis was more than 1, which indicated that the inhibition effect of the combination was synergism. Among them, the proportion of 4:6 had the strongest synergistic effect, and the toxicity ratio was 1.21.

关 键 词：解淀粉芽孢杆菌 玉米小斑病 脂肽粗提物 吡唑醚菌酯 协同作用 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]