钩吻醇提物对兔离体肠肌的舒张作用及其机制  

Relaxed effect of Gelsemium elegans Benth extraction by ethanol on intestinal smooth muscle of rabbit in vitro and its mechanism

作　　者：徐海云 王帅帅 谭成杰 胡菊芳 陈小军[1,2] XU Haiyun;WANG Shuaishuai;TAN Chengjie;HU Jufang;CHEN Xiaojun(College of Veterinary Medicine,Hunan Agricultural University,Changsha,Hunan 410128,China;Engineering Research Center of Veterinary Drugs of Hunan Province,Changsha,Hunan 410311,China)

机构地区：[1]湖南农业大学动物医学院,湖南长沙410128 [2]湖南省兽药工程中心,湖南长沙410311

出　　处：《湖南农业大学学报（自然科学版）》2020年第4期461-465,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家重点研发计划专项(2017YFD0501403);国家自然科学基金项目(31572563)。

摘　　要：采用BL–420E生物机能实验系统,观察钩吻醇提物对兔离体肠肌自主收缩及对工具药乙酰胆碱(Ach)、BaCl2和组胺(HA)所致痉挛性收缩的影响,并探讨肌浆网Ryanodine受体阻断剂钌红(RR)、三磷酸肌醇(IP3)受体阻断剂肝素钠(HP)和一氧化氮合酶抑制剂左旋硝基精氨酸甲酯(L–NAME)对钩吻醇提物作用的影响。结果表明:终质量浓度0.16~0.64 mg/mL钩吻醇提物可剂量依赖性抑制离体肠肌自主收缩频率和张力;终质量浓度0.32 mg/mL钩吻醇提物对BaCl2、HA诱导的离体肠肌收缩频率和张力具有显著抑制作用(P<0.05),对Ach诱导的离体肠肌的收缩频率具有显著抑制作用(P<0.05),对张力有极显著抑制作用(P<0.01);RR、HP和L–NAME对经钩吻醇提物预处理的离体肠肌的收缩频率和张力分别表现为无影响、显著增强和显著拮抗(P<0.05)。In this experiment, the BL-420 E biological function experiment system was used to investigate the effect of Gelsemium elegans Benth extraction by ethanol(GEBEE) on the independent contraction of isolated intestinal muscles in rabbits and the spasmodic contraction induced by Ach, BaCl2 and HA, and to explore the effect of sarcoplasmic reticulum Ryanodine receptor blocker RR, IP3 receptor blocker HP and nitric oxide synthase inhibitor L-NAME on GEBEE. The results showed that 0.16-0.64 mg/mL of GEBEE could inhibit the voluntary contraction frequency and tension of isolated rabbit intestinal muscles in a dose-dependent manner. The 0.32 mg/m L of GEBEE significantly inhibited the contraction frequency and tension of isolated rabbit intestinal muscles induced by BaCl2 and HA(P<0.05) and the contraction frequency of isolated rabbit intestinal muscles induced by Ach(P<0.05), while highly significantly inhibited the contraction tension of isolated rabbit intestinal muscles induced by Ach(P<0.01). For the contraction frequency and tension of isolated rabbit intestinal muscles pretreated with GEBEE, RR, HP and L-NAME showed no effect, significant enhancement and significant antagonism(P<0.05), respectively.

关 键 词：兔 离体肠肌 钩吻醇提物 舒张作用 工具药 阻断剂 抑制剂 

分 类 号：S816.7[农业科学—饲料科学] S859.797[农业科学—畜牧学]