虎杖有效成分对DN模型小鼠肾组织病理改变的影响  

Pathology effect of PolygonumCuspidatum’s effective components on kidneys of mice with diabetic nephropathy

作　　者：王达利 孟凤仙[2] 商学征[2] 王洁 张承承[2] 白华 卜祥伟 张正菊 刘豫玥[2] WANG Dali;MENG Fengxian;SHANG Xuezheng;WANG Jie;ZHANG Chengcheng;BAI Hua;BU Xiangwei;ZHANG Zhengju;LIU Yuyue(Community Health Service center in Yanshan of Fangshan District,Beijing 102500,China;Dongfang Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100078,China;Shunyi Hospital Affiliated to Beijing Hospital of Traditional Chinese Medicine,Beijing 101300,China;Guangwai Hospital of Xicheng District,Beijing 100053,China;Traditional Chinese Medicine Department of Xiamen University,Xiamen 361005,China)

机构地区：[1]北京市房山区燕山地区社区卫生服务中心,北京102500 [2]北京中医药大学东方医院,北京100078 [3]北京市顺义区中医院,北京101300 [4]北京市西城区广外医院,北京100053 [5]厦门大学医学院中医系,厦门361005

出　　处：《天津中医药大学学报》2020年第4期441-445,共5页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目资助(No.81273886)。

摘　　要：[目的]探讨虎杖有效成分对糖尿病肾病小鼠肾组织病理改变的影响。[方法]采用自发性2型糖尿病模型KK-Ay小鼠予高脂饲料诱导3周制备DN模型,造模成功后随机分为模型组,阳性药组,大黄素、虎杖苷高、中、低剂量组,每组各6只。另设6只C57BL/6J小鼠为正常组。每天灌胃给药1次,连续干预12周后处死小鼠,取肾脏行HE,PAS染色。光镜下观察肾组织病变并进行评分,PAS染色下测定肾小球基质相对面积。电镜下观察肾组织超微结构形态学改变。[结果]与模型组比较,虎杖苷高剂量组小鼠肾小管病变显著减轻(P<0.05);大黄素高剂量组,虎杖苷各剂量组小鼠肾小球基质相对面积显著降低(P<0.01,P<0.05);大黄素各剂量可改善DN模型小鼠肾小球病变,虎杖苷中剂量可明显改善DN模型小鼠肾组织超微结构病变,足细胞足突结构基本正常。[结论]大黄素、虎杖苷能不同程度的减轻DN模型小鼠肾组织病理损伤,对肾脏组织具有保护作用。[Objective]To investigated the pathology effect of PolygonumCuspidatum’s effective components on kidneys of mice with diabetic nephropathy(DN).[Methods]KK-Ay mice with spontaneous type 2 diabetes were fed with high-fat diets for 3 weeks to establish DN models.The successful models of mice were randomly divided into the model group,the positive drug group,high-dose,medium-dose and low-dose groups of emodin and high-dose,medium-dose and low-dose groups of polydatin.The C57BL/6J mice were recruited as the control group.Each group included 6 mice.Each mice was administered once daily.After twelve weeks of intervention,all mice were sacrificed and the kidneys were harvested for hematoxylin-eosin(HE)and periodic acid-schiff(PAS)stain.The renal lesions were classified and scored under light microscope.The relative area of mesangium extracellular matrix was measured under the PAS stain.Ultrastructural morphological changes of renal tissue were measured under electronmicroscope.[Results]Compared with the model group,the renal tubular lesions in the high dose of polydatin group were significantly reduced(P<0.05);Mesangium extracellular matrix relative area was significantly decreased in polydatin group and the high dose group of emodin(P<0.01,P<0.05).The pathological changes of glomerulus were diminished in emodin group.The structure of podocytefootprocess was normal in the medium dose group of polydatin which can significantly reduce the ultrastructural lesions of renal tissue.[Conclusion]Emodin and polydatin can significantly lighten the renal lesions of mice with DN and have a protective effect on kidney.

关 键 词：大黄素 虎杖苷 糖尿病肾病 肾组织病理 

分 类 号：R285.5[医药卫生—中药学]