疏肝法对小鼠卵母细胞质量的作用与BMP15/Smads信号通路的关系  

Effects of Shugan treatment on Bone morphogenetic protein-15 and Smads signal pathway in improving oocyte quality of the mice

作　　者：白静[1,2] 杜惠兰[1] 杨丽芸[1] BAI Jing;DU Hui-lan;YANG Li-yun(Hebei University of Chinese Medicine,Shijiazhuang Hebei,050200,China;Langfang Health Vocational College,Langfang Hebei 065001,China)

机构地区：[1]河北中医学院,河北石家庄050200 [2]廊坊卫生职业学院,河北廊坊065001

出　　处：《时珍国医国药》2020年第3期522-524,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81173294);河北省自然科学基金(H2015423050)。

摘　　要：目的研究疏肝法对小鼠卵母细胞中BMP-15、BMPRII、ALK6、Smad1/5/8表达的影响,进一步揭示疏肝法提高控制性超排卵小鼠卵母细胞质量的作用机制。方法将200只雌性昆明小鼠随机分为四组:空白对照组、COH组、疏肝低剂量组和疏肝高剂量组,每组50只。按1ml/100g体重进行灌胃,空白对照组及COH组使用蒸馏水灌胃,疏肝低剂量组和高剂量组分别使用逍遥丸0.3g/ml、0.6g/ml灌胃,每日1次,连续10天,第11日疏肝低、高剂量组及COH组均腹腔注射孕马血清促性腺激素,48h后均腹腔注射人绒毛膜促性腺激素,16h后处死小鼠,获取MⅡ期卵母细胞,检测BMP-15、BMPRII、ALK6、Smad1/5/8蛋白及mRNA表达。结果与空白对照组比较,COH组BMP-15、BMPRII、ALK6、Smad1/5/8蛋白及mRNA表达无统计学意义(P>0.05);疏肝低、高剂量组BMP-15、ALK6、Smad1/5/8蛋白及mRNA表达增强(P<0.05);疏肝高剂量组BMPRII蛋白及mRNA表达增强(P<0.05)。结论疏肝法可上调小鼠卵母细胞中BMP-15的表达,诱导其与Ⅱ型受体BMPRⅡ结合,进一步活化ALK6,从而启动信号传导,发挥影响小鼠卵母细胞质量的作用。Objective To study the effects of Shugan treatment on Bone morphogenetic protein-15(BMP-15),BMPRII,ALK6,Smad1/5/8 in superovulated mice.Methods We divided 200 mice into 4 groups randomly:control group,COH group,Shugan low dose group and high dose group(N=50).The mice in each group were gavaged with 1 ml/100 g for 10 days consecutively.We drenched the control group and the COH group with distilled water.Shugan low and Shugan high dose group were given 0.3 g/ml and 0.6 g/ml refined oral solution of Xiaoyao Pill.The COH group and the mice in treatment groups were dealt with intraperitoneal injection of gonadotropin from pregnant.After 48 hours,they were dealt with intraperitoneal injection of human chorionic gonadotropin.The mice were killed after 16 hours to obtain the MII oocytes.The expressions of BMP-15,BMPRII,ALK6,Smad1/5/8 in oocytes were detected with immunohistochemistry method.The expressions of BMP-15,BMPRII,ALK6,Smad1/5/8 mRNA in oocytes were detected with reverse transcription-polymerase chain reaction.Results Compared with the control group,the protein and mRNA expressions of BMP-15,BMPRII,ALK6,Smad1,5 and 8 in the COH group were no differences(P>0.05);the protein and mRNA expressions of BMP-15,ALK6,Smad1/5/8 in Shugan low group and Shugan high group were increased(P<0.05);the protein and mRNA expressions of BMPR II in the Shugan high dose group were increased significantly(P<0.05).Conclusion Shugan treatment can raise the expression of BMP-15 in the mouse oocytes,and may combine with the second type of receptor BMPRⅡ,then activated the receptor ALK6,and started the signal transduction of Smads pathway to improve the quality of oocytes.

关 键 词：逍遥丸 卵母细胞 BMP-15 BMPRII ALK6 SMAD1 SMAD5 Smad8 

分 类 号：R285.5[医药卫生—中药学]