管灸对面神经损伤模型家兔面神经超微结构及蛋白ERK1/2、p-ERK1/2、c-jun表达的影响  

Effect of Guan-moxibustion on Ultrastructure of facial nerve and the Expression of ERK1/2、p-ERK1/2、c-jun in Rabbits with Facial Nerve Injury

作　　者：陈静[1] 宋军[2] 田丰玮 肖雪[1] 张献文 杨金蓉[1] CHEN Jing;SNG Jun;TIAN Feng-wei;XIA Xue;ZHANG Xian-wen;YANG Jin-rong(Chengdu University of Traditonal Cinese Medcine,Chengdu Sichuan 610072,China;Sichuan Academy of Chinese Medicine Sciences,Chengdu Sichuan 610041,China;Chongqing Institute of Traditoal Chinese Medcine,Chongqing 400013,China)

机构地区：[1]成都中医药大学,四川成都610072 [2]四川省中医药科学院,四川成都610041 [3]重庆市中医研究院,重庆渝中400013

出　　处：《时珍国医国药》2020年第3期734-736,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81373740);全国中医优秀人才研修项目【2017】124。

摘　　要：目的探讨管灸促进损伤面神经修复再生的分子机制。方法建立家兔面神经压榨损伤模型,观察管灸对家兔标记损伤段面神经超微结构及蛋白ERK1/2、p-ERK1/2、c-jun表达的影响。结果①面神经超微结构:模型组脱髓鞘较重,雪旺氏细胞内线粒体肿胀程度重;管灸组轻度脱髓鞘,雪旺氏细胞线粒体肿胀较轻;热疗组脱髓鞘病变和线粒体肿胀度介于模型组和管灸组之间。②ERK1/2蛋白表达:管灸组较模型组明显升高(P<0.05);假手术组及热疗组较模型组均无明显改变(P>0.05);③p-ERK1/2蛋白表达:管灸组、假手术组较模型组均明显升高(P<0.01或P<0.05);热疗组较模型组无明显改变(P>0.05);④c-jun蛋白表达:管灸组较模型组明显升高(P<0.05);假手术组及热疗组较模型组均无明显改变(P>0.05)。结论面神经压榨损伤后家兔面神经ERK1/2活化水平降低,管灸能提高ERK1/2通路的活性,促进核蛋白c-jun的表达,维持损伤面神经雪旺氏细胞的存活,以促进面神经损伤的修复。Objective To reveal the molecular mechanism of Guan-moxibustion in promoting the repair and regeneration of facial neuritis.Methods We established animal models of injury of facial nerve with rabbits,the marked segment of facial nerve was taken from each group of rabbits to observe the ultrastructure and detect the protein expression of erk1/2,phosphorylated erk1/2(p-erk1/2)and c-jun.Results①Ultrastructure of facial nerve:the demyelination of model group was serious,the mitochondria swelling of Schwann cells was serious;the mild demyelination and the mitochondria swelling of Schwann cells were mild in Guan-moxibustion group;the demyelination lesion and mitochondria swelling of Schwann cells were mild in the degree of granulosa swelling was between the model group and Guan-moxibustion group.②The expression level of erk1/2 protein showed that:Comparing with the model group,Guan-moxibustion group increased significantly(P>0.05).There was no significant difference between the model group with sham operation group or hyperthermia group(P>0.05).③The expression level of p-erk1/2 protein showed that:Comparing with the model group,Guan-moxibustion group or sham operation group increased significantly(P<0.01).And there was no significant difference between the model group with hyperthermia group(P>0.05).④The expression of c-jun showed that:Comparing with the model group,Guan-moxibustion group increased significantly(P<0.05).And there was no significant difference between the model group with hyperthermia group or sham operation group(P>0.05).Conclusion The activation level of erk1/2 protein in rabbits with facial nerve injury was decreased.Guan-moxibustion can improve the activity oferk1/2 pathway,promote the expression of nuclear protein c-Jun,maintain the survival of Schwann cells of injured facial nerve,and promote the repair of facial nerve injury.

关 键 词：管灸 面神经损伤 超微结构 细胞外调节蛋白激酶1/2 磷酸化细胞外调节蛋白激酶1/2 C-JUN 

分 类 号：R245.8[医药卫生—针灸推拿学]