反向-HPLC法同时检测复方益肝丸3种活性成分的含量  

Simultaneous determination of three active components in compound Yigan Pills by reverse-high performance liquid chromatography

作　　者：李业雨 刘世萍[1] 马妍妍[1] 海鑫 LI Ye-yu;LIU Shi-ping;MA Yan-yan;HAI Xin(Department of Pharmacy,First Affiliated Hospital of Harbin Medical University,Harbin 150001,China)

机构地区：[1]哈尔滨医科大学附属第一医院药学部,黑龙江哈尔滨150001

出　　处：《实用药物与临床》2020年第8期748-751,共4页Practical Pharmacy and Clinical Remedies

摘　　要：目的建立复方益肝丸同时检测丹皮酚、大黄素和大黄酸3种活性成分含量的高效液相色谱检测方法。方法复方益肝丸经研钵研细,取约0.5 g,精密称定,甲醇溶解,超声提取,乙腈与0.1%磷酸溶液梯度洗脱,Agilent ZORBAX SB-C 18色谱柱分离,柱温30℃,外标法定量检测。结果丹皮酚、大黄素和大黄酸在0.3~30.0μg/ml浓度范围内具有良好的线性关系;相关系数r均大于0.995;检测限:丹皮酚0.075μg/ml、大黄酸0.091μg/ml、大黄素0.032μg/ml;加标回收率94.3%~104.3%;精密度(n=6)<5.0%;重复性(n=6)<5.0%。结论该方法具有简便、快速、重复性好、准确等优点,可用于复方益肝丸中活性成分丹皮酚、大黄素和大黄酸含量的测定。Objective To establish an HPLC method for the simultaneous determination of paeonol,emodin and rhein in compound Yigan Pills.Methods The compound Yigan Pill was refined by mortar,0.5 g of which was taken,precisely weighed,dissolved in methanol,and extracted by ultrasound;then it was eluted by acetonitrile and 0.1%phosphoric acid solution gradient,separated by Agilent ZORBAX SB-C 18 column at 30℃,and quantitatively detected by external standard method.Results Paeonol,emodin and rhein showed good linear relationships in the range of 0.3~30.0μg/ml,and correlation coefficients(r)were all above 0.995.Detection limits:paeonol 0.075μg/ml,emodin 0.091μg/ml,rhein 0.032μg/ml.Recoveries of paeonol,emodin and rhein were in the range of 94.3%~104.3%;the relative standard deviation of precision and repeatability were within 5.0%(n=6).Conclusion The method is simple,quick,reproducible and accurate,which can be applied for determining the contents of paeonol,emodin and rhein in compound Yigan Pills.

关 键 词：高效液相色谱 复方益肝丸 丹皮酚 大黄素 大黄酸 

分 类 号：R286.0[医药卫生—中药学]