两种乳菇菌丝生长的最适培养基与菌根合成  

Optimum media for hyphal growth and mycorrhizal synthesis of two Lactarius species

作　　者：王冉[1,2] Guerin-Laguette Alexis 于富强[1,2] WANG Ran;Guerin-Laguette Alexis;YU Fu-Qiang(CAS Key Laboratory for East Asia Biodiversity and Biogeography,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,Yunnan 650201,China;SWFU-KIB CAS Joint Institute for Applied Mycology,Kunming,Yunnan 650224,China;The New Zealand Institute of Plant and Food Research Limited,Christchurch 8053,New Zealand)

机构地区：[1]中国科学院昆明植物研究所东亚植物多样性与生物地理学重点实验室,云南昆明650201 [2]西南林业大学-中国科学院昆明植物研究所联合应用真菌研究所,云南昆明650224 [3]新西兰植物与食品研究所,新西兰基督城8053

出　　处：《菌物学报》2020年第7期1346-1355,共10页Mycosystema

基　　金：国家自然科学基金(31901204);中国科学院昆明植物研究所引进人才项目(Y9627111K1)。

摘　　要：通过测量菌丝直径和干重对松乳菇(rcb-74)和靓丽乳菇(rll-107,rmsh-118)进行最适菌丝生长培养基的筛选。结果表明所有菌株均在改良的biotin-aneurine-folic acid(BAF)培养基上表现出较大的菌丝直径以及最大菌丝干重。用改良的BAF培养基制备乳菇液体菌丝,接种云南松、马尾松和油松,并在13–30d后发现rcb-74与云南松和油松形成菌根,rll-107与马尾松形成菌根,rmsh-118与云南松形成菌根。Optimum culture medium for Lactarius deliciosus rcb-74,L.vividus rll-107 and L.vividus rmsh-118 was screened by measuring diameter and dry weight of mycelia.The results showed that all isolates had relatively large diameter of mycelia and the maximum dry weight on modified biotin-aneurine-folic acid(BAF)medium.Lactarius mycelium cultured in modified BAF liquid medium was inoculated to Pinus yunnanensis,P.massoniana and P.tabuliformis,and mycorrhizae were observed in combinations of L.deliciosus rcb-74 with P.yunnanensis and P.tabuliformis,combination of L.vividus rll-107 with P.massoniana,as well as combination of L.vividus rmsh-118 with P.yunnanensis in 13–30 days.

关 键 词：乳菇 培养基 菌根合成 

分 类 号：S646[农业科学—蔬菜学]