赤霉属真菌诱导龙血竭形成的HPLC指纹图谱  

HPLC characteristic fingerprints of resina draconis induced by Gibberella sp.

作　　者：曾旭[1] 孙睿 陈晓梅[1] 郭顺星[1] ZENG Xu;SUN Rui;CHEN Xiao-Mei;GUO Shun-Xing(Institute of Medicinal Plant Development,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100193,China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《菌物学报》2020年第7期1421-1428,共8页Mycosystema

基　　金：中国医学科学院医学与健康科技创新工程(2017-I2M-3-013)。

摘　　要：龙血竭为百合科植物龙血树所产的树脂类药材,常常因树干部位受损伤后,经微生物侵染并分泌树脂,经提而成.本研究以活性菌株诱导所得龙血竭药材为研究对象,采用HPLC指纹图谱与对照药材相比较,为其质量控制提供评价及分析方法.采用ZORBAX SB-C18色谱柱(5μm,4.6mm×250mm),流动相A为30%乙腈+0.3%乙酸,流动相B为100%乙腈,梯度洗脱,流速1.0mL/min,检测波长278nm与309nm,柱温25℃.结果 发现,各龙血竭样品指纹图谱与对照样品相似度高,确定14个共有峰,并通过对照指认了7,4'-二羟基黄酮、龙血素A、龙血素B和白藜芦醇共4个特征性化学成分.本研究发现活性真菌菌株诱导产物可作为天然龙血竭的替代品,诱导药材质量佳,诱导效果好.Resina draconis is a resinous medicine secreted by Dracaena sp.(Liliaceae)resulted from physical damage and fungal induction.In this study,HPLC fingerprints of resina draconis induced by fungi were established to provide evaluation and analysis methods for quality control of resina draconis.Using ZORBAX SB-C18 column(5μm,4.6 mm×250 mm)with mobile phase A of 30%acetonitrile+0.3%glacial acetic acid,and mobile phase B of 100%acetonitrile,separation was carried out by gradient elution.The flow rate was 1.0 mL/min,the column temperature was 25°C,and the detective wavelengths were 278 nm and 309 nm.The similarity between the fingerprints of resina draconis sample and the reference sample was high.In total,14 characteristic peaks were selected,and four major characteristic ingredients,7,4’-dihydroxyfl avone,loureirin A,loureirin B and resveratrol,in resina draconis were detected.The active fungus-induced products with good quality could be used as substitute for natural resina draconis,and this induction technique seems effective as compared with the natural depletive process.

关 键 词：龙血竭 真菌 指纹图谱 龙血素 

分 类 号：R284.1[医药卫生—中药学]