HBV-ACLF患者肝脏B7-H6的表达水平与肝损伤的相关性  

Correlation between the expression level of hepatic B7-H6 and liver injury in HBV-ACLF patients

作　　者：袁青[1] 杨小安[2] 兰天云[3] 邹勇[1] Yuan Qing;Yang Xiaoan;Lan Tianyun;Zou Yong(Department of Blood Transfusion,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China)

机构地区：[1]中山大学附属第三医院输血科,广州510630 [2]中山大学附属第三医院感染科,广州510630 [3]中山大学附属第三医院中心实验室,广州510630

出　　处：《新医学》2020年第8期601-604,共4页Journal of New Medicine

基　　金：广东省科技计划项目(2016A020215215)。

摘　　要：目的探讨HBV相关慢加急性肝衰竭(HBV-ACLF)患者肝组织自然杀伤细胞天然细胞毒性受体NKp30特异性配体B7同源物6(B7-H6)的表达水平与肝损伤的相关性。方法收集HBV-ACLF患者肝移植术中切除的肝组织(HBV-ACLF组)及CHB患者(CHB组)肝穿刺标本各18例,并以5例来源于肝脏血管瘤切除标本旁的正常肝组织为正常对照组。利用免疫组织化学染色检测3组肝组织中B7-H6的表达水平。分析肝组织B7-H6表达水平及与肝损伤程度(总胆红素水平)的相关性。结果B7-H6主要表达在肝细胞,而且多在肝小炎症反应强烈区域内。定量分析显示,HBV-ACLF组肝组织B7-H6表达的平均相对密度(127.02±40.25)高于轻度CHB组(22.08±10.82)和正常对照组(14.26±5.67)(P均<0.05),轻度CHB组与正常对照组比较差异无统计学意义(P>0.05)。HBV-ACLF患者肝脏B7-H6水平与血清总胆红素水平呈正相关(rs=0.604,P<0.05)。结论肝脏B7-H6高表达可能是NKp30/B7-H6被激活导致HBV-ACLF免疫性肝损伤加剧,肝细胞大量凋亡和坏死的重要因素。Objective To investigate the correlation between the expression level of hepatic B7 homologue 6(B7-H6) and liver injury in patients with hepatitis B virus-related acute-on-chronic liver failure(HBV-ACLF).Method Needle biopsy liver tissues and resected liver tissue samples during liver transplantation were obtained from patients with mild chronic hepatitis B(CHB) and HBV-ACLF patients,respectively.Normal liver tissues from 5 cases of hemangioma were utilized as normal controls.The expression levels of hepatic B7-H6 were quantitatively measured by immunohistochemical staining.The correlation between the expression of B7-H6 and the degree of liver injury(total bilirubin level) was analyzed.Results B7-H6 was mainly expressed in liver cells,especially in the area with intense hepatic inflammatory responses.Quantitative analysis demonstrated that the average relative density of B7-H6 expression in the HBV-ACLF group was(127.02±40.25)/lpf,significantly higher compared with(22.08±10.82)/lpf and(14.26±5.67)/lpf in the mild CHB and normal control groups(both P < 0.05).No statistical significance was noted between the mild CHB and normal control groups(P > 0.05).In the HBV-ACLF patients,the B7-H6 expression level was positively correlated with the serum total bilirubin level(rs = 0.604,P < 0.05).Conclusion High expression of hepatic B7-H6 is probably a critical factor leading to aggravation of immunitymediated liver injury induced by NKp30/B7-H6 activation,the apoptosis and necrosis of a large quantity of liver cells in HBV-ACLF patients.

关 键 词：乙型肝炎病毒 肝衰竭 自然杀伤细胞 B7同源物6 

分 类 号：R512.62[医药卫生—内科学] R575[医药卫生—临床医学]