水稻白叶枯病感病相关基因Xig1的分子鉴定及抗病资源创制  

Molecular identification of rice bacterial blight susceptible gene Xig1 and creation of disease resistant resources

作　　者：郑凯丽 纪志远[1] 郝巍[1] 唐永超 韦叶娜 胡运高[2] 赵开军[1] 王春连[1] ZHENG Kai-Li;JI Zhi-Yuan;HAO Wei;TANG Yong-Chao;WEI Ye-Na;HU Yun-Gao;ZHAO Kai-Jun;WANG Chun-Lian(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement,Beijing 100081,China;Rice Research Institute,Southwest University of Science and Technology,Mianyang 621010,Sichuan,Ch)

机构地区：[1]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京100081 [2]西南科技大学水稻研究所,四川绵阳621010

出　　处：《作物学报》2020年第9期1332-1339,共8页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31571640);中国科协青年人才托举工程(2017QNRC001)资助。

摘　　要：水稻白叶枯病相关基因Xig1在感病亲本IR24中受白叶枯病菌的诱导表达。本研究通过克隆与比较Xig1的序列后发现,抗病野生稻导入系W6023与感病亲本IR24中Xig1等位基因的差异主要集中在启动子区。水稻原生质体观察XIG1在细胞中的定位,显示XIG1定位于细胞质中。利用CRISPR/Cas9系统对感病籼稻品种IR24的Xig1靶点进行基因组编辑,获得并评价了多个Xig1定点突变株系对白叶枯菌的抗性。与野生型相比,8个IR24的Xig1基因定点突变株系对水稻白叶枯病的抗性得到明显提高,而农艺性状无显著差异。Xig1是新发现的水稻白叶枯病感病基因,该基因在水稻白叶枯病感病性中贡献的研究,不仅为创制新的抗病资源提供理论指导,还将丰富和加深我们对植物先天免疫系统的认知。The rice bacterial blight susceptible gene Xig1(Xoo-induced-gene 1),was found highly induced by Xanthomonas oryzae pv.oryzae(Xoo)in susceptible parent line IR24 but not in introgression line W6023.In this study,we cloned and sequenced Xig1 alleles isolated from W6023 and IR24,and the main difference in nucleotide sequences between Xig1 alleles of W6023 and IR24 was in the promoter region.Subcellular localization in rice protoplast indicated that XIG1 localized to cytoplasm.CRISPR/Cas9-based genome editing technologies were also applied to knock out Xig1 in IR24.Eight Xig1-edited mutant lines showed a higher resistance to Xoo,and all agronomic traits surveyed are consistent.Xig1 is a novel susceptible gene for bacterial blight in rice.The studying of the mechanism of Xig1 to Xoo in rice will not only provide a theorical guidance for disease resistance resource,but also benefit the understanding of plant innate immunity.

关 键 词：水稻 白叶枯病 Xig1 CRISPR/Cas9 

分 类 号：S435.111.47[农业科学—农业昆虫与害虫防治]