玉米ZmCIPK24-2基因在盐胁迫应答中的功能研究  

Functional analysis of ZmCIPK24-2 gene from maize in response to salt stress

作　　者：李健 王逸茹[2] 张凌霄 孙明昊 秦阳 郑军 LI Jian;WANG Yi-Ru;ZHANG Ling-Xiao;SUN Ming-Hao;QIN Yang;ZHENG Jun(College of Agronomy,Jilin Agricultural University,Changchun 130118,Jilin,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China)

机构地区：[1]吉林农业大学农学院,吉林长春130118 [2]中国农业科学院作物科学研究所,北京100081 [3]北京农学院植物科学技术学院,北京102206

出　　处：《作物学报》2020年第9期1351-1358,共8页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2016YFD0101002);中国农业科学院创新工程专项经费资助。

摘　　要：土壤盐化会影响作物正常生长发育,导致农作物减产。植物在长期适应环境的过程中,进化出了相应的耐盐分子机制。钙调磷酸酶B类蛋白(CBL)及CBL互作蛋白激酶(CIPK)参与植物对盐胁迫的响应。本研究鉴定到一个拟南芥AtSOS2的同源基因ZmCIPK24-2,实时荧光定量聚合酶链式反应结果表明ZmCIPK24-2基因在玉米各组织部位广泛表达,其中在花粉表达量最高;ZmCIPK24-2受盐胁迫诱导表达。ZmCIPK24-2能部分互补拟南芥atsos2突变体的盐敏感表型,在高盐浓度下转基因株系比atsos2突变体的存活率显著提高,根长显著增长。亚细胞定位实验表明ZmCIPK24-2定位于细胞质、细胞膜与核膜。利用酵母双杂交实验及LUC互补成像实验发现ZmCIPK24-2与玉米CBLs家族中的ZmCBL1、ZmCBL4、ZmCBL8和ZmCBL9互作。本研究为解析玉米CBL-CIPK信号通路的功能提供了新的实验证据。Soil salinity affects the normal growth and development of crops,resulting in reduced crop yields.In the process of long-term adaptation,plants have evolved targeted salt-resistant molecular mechanisms.The calcineurin B-like protein(CBL)and CBL-interaction protein kinase(CIPK)are involved in plant response to salt stress.In this study,we identified an AtSOS2 homologous gene ZmCIPK24-2.The results of real time-qPCR showed that ZmCIPK24-2 gene was ubiquitously expressed in maize,especially in pollen,and was induced by salt stress.It was found that ZmCIPK24-2 could partially complement the salt-sensitive phenotype of the atsos2 mutant.The survival rate and the root length of ZmCIPK24-2-overexpressed lines were significantly increased compared with atsos2 mutant under high salt concentration.Subcellular localization experiments showed that ZmCIPK24-2 was localized in cytoplasm,cell membrane,and nuclear membrane.Yeast two-hybrid experiments and LUC complementation imaging assays showed that ZmCIPK24-2 was interacted with ZmCBL1,ZmCBL4,ZmCBL8,and ZmCBL9,respectively.This study provides a new experimental evidence for the functional analysis of CBL-CIPK signaling pathway in maize.

关 键 词：CIPK CBL 耐盐性 玉米 

分 类 号：S513[农业科学—作物学]