不同剂量龙血生肌温敏凝胶对失神经支配创面促愈合的实验研究  

Experimental Study of Different Doses of Longxueshengji Thermo-sensitive Gel on Healing of Denervated Wounds

作　　者：董黎强 罗统富 金圣煊 魏浩 刘启明 黄余亮 安忠诚 DONG Li-qiang;LUO Tong-fu;JIN Sheng-xuan(Department of Orthopaedics,Second Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine,Hangzhou(310005),China)

机构地区：[1]浙江中医药大学附属第二医院,杭州310005 [2]桐乡市第二人民医院,嘉兴314500 [3]杭州市创新中药标准化研究所有限公司,杭州310005 [4]杭州市富阳中医骨伤医院,杭州311400

出　　处：《中国中西医结合外科杂志》2020年第4期595-600,共6页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：浙江省科技厅研究项目(2017C33087);浙江省中管局研究项目(2017ZA066)。

摘　　要：目的:观察应用不同剂量的龙血生肌温敏凝胶对大鼠失神经支配创面促愈合的影响,明确最佳剂量。方法:健康SD大鼠80只,建立失神经支配创面动物模型,随机分成4组,高剂量组、中剂量组、低剂量组、对照组各20只,高、中、低剂量组分别用相应剂量(0.8、0.4、0.2 g/mL)的龙血生肌温敏凝胶,对照组使用凡士林纱布外敷创面,观察创面愈合的大体情况,计算创面愈合所需的时间及其愈合率,采用HE染色检测各时间点创面组织切片的成纤维细胞数量。结果:高、中、低剂量组及对照组创缘皮肤血供均良好,无明显红肿发炎及坏死征兆;创面愈合时间及愈合率对比,高剂量组及中剂量组相近,均比低剂量组及对照组优(P <0.05),而低剂量组又比对照组愈合快(P <0.05)。高中低剂量组的成纤维细胞数量在5个时间点均比对照组显著增多(P <0.05),高、中剂量组的成纤维细胞数量在造模后第11天比低剂量组显著增多(P <0.05)。结论:龙血生肌温敏凝胶对失神经支配创面有促愈合作用,中高剂量的龙血生肌温敏凝胶疗效最佳。Objective The aim of this study was to observe the effect of different doses of Longxueshengji thermo-sensitive gelon the healing of denervated wounds in rats and to determine the optimal dose. Methods Eighty healthy SD rats were established modeling of denervated wounds. They were randomly divided into 4 groups: high-dose group, medium-dose group, low-dose group and control group respectively. The high, middle and low dose groups were respectively dosed the Longxueshengji thermo-sensitive gel. The control group were treated with Vaseline gauze externally to observe the general condition of wound healing. The time required for wound healing and its healing rate were calculated. Results Those wounds were good, no obvious signs of redness, inflammation and necrosis. The wound healing time and rate were similar, and the high dose group and the middle dose group were similar, which were faster than those of the low dose group and the control group(P<0.05). The low-dose group healed faster than that of the control group(P<0.05). Conclusion The Longxueshengji thermo-sensitive gel has a healing effect on the enervated wound and the middle dose was the best one.

关 键 词：温敏凝胶 失神经支配创面 中药剂量 

分 类 号：R285.5[医药卫生—中药学]