壳聚糖/聚丙烯酸多层膜动态微孔结构的构建及应用  

Construction and Application of Dynamic Microporous Structure of Chitosan/Polyacrylic Polyelectrolyte Multilayer Films

作　　者：孙一新 王伟[1,2] 盛扬 周超 周俊涛 张嵘 SUN Yixin;WANG Wei;SHENG Yang;ZHOU Chao;ZHOU Juntao;ZHANG Rong(Jiangsu Key Laboratory of Environmentally Friendly Polymeric Materials,Changzhou University,Changzhou 213164,China;School of Materials Science and Engineering,Changzhou University,Changzhou 213164,China;Institute of Biomedical Engineering and Health Science,Changzhou University,Changzhou 213164,China)

机构地区：[1]常州大学江苏省环境友好高分子材料重点实验室,江苏常州213164 [2]常州大学材料科学与工程学院,江苏常州213164 [3]常州大学生物医学工程与健康科学研究院,江苏常州213164

出　　处：《常州大学学报（自然科学版）》2020年第4期10-18,45,共10页Journal of Changzhou University：Natural Science Edition

基　　金：国家自然科学基金地区基金资助项目(515630036)。

摘　　要：传统多层膜包载疏水药物通常涉及合成并且步骤繁琐,因此,有必要开发一种快速、简单的负载疏水性药物的方法。采用层层自组装方法构建壳聚糖/聚丙烯酸(CS/PAA)n多层膜,并用石英晶体微天平(QCM)成功跟踪(CS/PAA)n多层膜的组装。扫描电镜(SEM)结果显示经过酸处理多层膜形成孔洞结构,在100%相对湿度下(CS/PAA)n多层膜自发愈合。利用毛细作用将疏水性药物四环素负载到多孔膜内,在100%相对湿度条件下对微孔进行封闭,药物分子被包裹在膜内,实现了疏水性药物的大量负载。将载药多层膜在体外进行药物释放模拟,释放时间超过400 h,实现了药物的长效缓释。最后将载药多层膜进行了大肠杆菌和金黄色葡萄球菌的抗菌实验,显示了载药多层膜良好的长效抗菌性能。Multilayer films loading hydrophobic drugs usually involve synthesis and the steps are complexmplex.Therefore,we have developed a fast and simple method for multilayer films loading hydrophobic drugs.In this study,(CS/PAA)n multilayer film was constructed by layer-by-layer self-assembly method and characterized by quartz crystal microbalance(QCM).Scanning electron microscopy(SEM)results showed that the acid-treated(CS/PAA)n multilayer film form porous structure and can spontaneously heal at 100%relative humidity.The hydrophobic drug tetracycline is loaded into the porous film by capillary action,and the micropores are blocked under the condition of 100%relative humidity,resulting in the drug molecules being encapsulated in the film and realizing a large amount of loading of the hydrophobic drug.The drug-loaded multilayer film was released in phosphate buffer solution for more than 400 h,which achieved long-term sustained release of the drug.Finally,the drug-loaded multilayer films were performed to antibacterial experiments of Escherichia coli and Staphylococcus aureus,showing good long-time antibacterial properties.This method of using acid-treated to make a pore-humidity healing properties for drug loading and sustained release provides a new idea in the field of drug release.

关 键 词：层层自组装 多孔海绵结构 湿度响应 动态转变 药物释放 

分 类 号：TK[动力工程及工程热物理]