脂质体二甲基姜黄素体内体外抑制大鼠脾淋巴细胞增殖并调节细胞周期  

Liposomal Dimethyl Curcumin Inhibits Cell Proliferation and Reg-ulates Cell Cycle of Rat Spleen Lymphocytes in vivo and in vitro

作　　者：孙芝源 郭亚鸣 戈兴 陆晨曦 邵丹迪 周晓鹰[1] SUN Zhiyuan;GUO Yaming;GE Xing;LU Chenxi;SHAO Dandi;ZHOU Xiaoying(School of Pharmaceutical Engineering&Life Sciences,Changzhou University,Changzhou 213164,China)

机构地区：[1]常州大学制药与生命科学学院,江苏常州213164

出　　处：《常州大学学报（自然科学版）》2020年第4期63-70,共8页Journal of Changzhou University：Natural Science Edition

基　　金：常州大学高层次人才引进启动基金(ZMF14020066);常州大学大学生创新创业基金暨“挑战杯-卓越”计划项目(Z13)。

摘　　要：二甲基姜黄素是姜黄素的一种结构衍生物,具有抗炎抗肿瘤作用,具有缓释作用的新剂型脂质体二甲基姜黄素(Lipo-DiMC)有较好的生物利用度和代谢稳定性。淋巴细胞的过度增殖、细胞周期紊乱和细胞凋亡异常是类风湿疾病的病理特征。采用薄膜法制备小粒径(0.2μm)的Lipo-DiMC、建立胶原诱导的关节炎(CIA)大鼠模型(正常组,CIA模型组和CIA+Lipo-DiMC治疗组)和体外细胞模型,探讨Lipo-DiMC对大鼠脾淋巴细胞增殖和淋巴细胞周期的影响。Lipo-DiMC关节腔注射治疗CIA大鼠(120μg/mL,每3 d关节腔注射治疗一次,每次400μL,共治疗6次)。在CIA发病进程中和Lipo-DiMC治疗过程中,对关节炎严重程度进行生理评估和指数评分;血常规检测外周血中各免疫细胞数量的变化。新鲜提取的大鼠脾淋巴细胞建立体外细胞模型,MTT法检测Lipo-DiMC对大鼠脾淋巴细胞增殖作用,细胞流式仪检测Lipo-DiMC对脾淋巴细胞周期作用。实验结果显示Lipo-DiMC在体内和体外均对大鼠脾淋巴细胞的细胞周期有调节作用。Dimethyl curcumin(DiMC)is one analogue of curcumin with anti-inflammatory property.Liposome dimethyl curcumin(Lipo-DiMC)has improved bioavailability,metabolic-stability and slow-release compared with DiMC.The excessive lymphocyte proliferation is one of characteristic of Rheumatoid arthritis(RA).The study is to investigate the effects of Lipo-DiMC on proliferation and cell cycle of rat spleen lymphocytes.Lipo-DiMC(particle size 0.2μm)was prepared by a thin film method.Collagen-induced arthritis(CIA)rat model was established(normal group,CIA model group and CIA+Lipo-DiMC treatment group)successfully.CIA rats were treated with Lipo-DiMC by intra-articular injection(120μg/mL,treated once every 3 d,400μL each time,6 treatments in total).Physiological assessment and index scores of arthritis severity were monitored over the CIA process and treatments;the dynamic change of cells number in peripheral blood of CIA rats and Lipo-DiMC treated rats were recorded by Routine blood test.In vivo cell cycle of spleen lymphocytes of normal,CIA and Lipo-DiMC treated CIA rats were detected by flow cytometry(PI staining).Freshly extracted rat spleen lymphocytes were used to established cell model to study the anti-proliferative and cell cycle regulatory effects of Lipo-DiMC in vitro.MTT assay was used to detect the proliferation rate of rat spleen lymphocytes with or without Lipo-DiMC added.Cell flow cytometry was used to detect the effect of Lipo-DiMC on cell cycle of spleen lymphocyte in vitro.Experiments showed that Lipo-DiMC demonstrated a regulatory effect on cell cycle of rat spleen lymphocyte in vitro and in vivo.

关 键 词：脂质体二甲基姜黄素 胶原诱导关节炎大鼠 淋巴细胞 细胞增殖 细胞周期 

分 类 号：R562.1[医药卫生—呼吸系统]