Rapid and sensitive detection of COVID-19 using CRISPR/Cas12a-based detection with naked eye readout, CRISPR/Cas12a-NER  

基于CRISPR/Cas12a的可视荧光法快速灵敏检测新型冠状病毒

作　　者：Xinjie Wang Mingtian Zhong Yong Liu Peixiang Ma Lu Dang Qingzhou Meng Wenwei Wan Xiaodong Ma Jia Liu Guang Yang Zifeng Yang Xingxu Huang Ming Liu 王鑫杰;钟名天;刘勇;马培祥;党露;孟庆洲;万闻蔚;马晓冬;刘佳;杨光;杨子峰;黄行许;刘明(Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University,Shanghai 201210,China;Institute for Brain Research and Rehabilitation,Guangdong Key Laboratory of Mental Health and Cognitive Science,Center for Studies of Psychological Application,South China Normal University,Guangzhou 510631,China;State Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital,Guangzhou Medical University,Guangzhou 510120,China;Kingmed Virology Diagnostic&Translational Center,Guangzhou Kingmed Center for Clinical Laboratory Co.,Ltd.,Guangzhou 510005,China;Affiliated Cancer Hospital&Institute of Guangzhou Medical University,Guangzhou 510095,China;School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China)

机构地区：[1]Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University,Shanghai 201210,China [2]Institute for Brain Research and Rehabilitation,Guangdong Key Laboratory of Mental Health and Cognitive Science,Center for Studies of Psychological Application,South China Normal University,Guangzhou 510631,China [3]State Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital,Guangzhou Medical University,Guangzhou 510120,China [4]Kingmed Virology Diagnostic&Translational Center,Guangzhou Kingmed Center for Clinical Laboratory Co.,Ltd.,Guangzhou 510005,China [5]Affiliated Cancer Hospital&Institute of Guangzhou Medical University,Guangzhou 510095,China [6]School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China

出　　处：《Science Bulletin》2020年第17期1436-1439,M0003,共5页科学通报（英文版）

基　　金：This work was supported by internal grants from Shanghai Tech University;State Key Laboratory of Respiratory Diseases;Shanghai Local Grants;National Clinical Research Center for Respiratory Disease;Guangzhou Institute of Respiratory Health;the First Affiliated Hospital of Guangzhou Medical University。

摘　　要：The outbreak of the 2019 novel coronavirus disease(COVID-19)has had a significant impact on global health.To advance the diagnostic method of COVID-19,we developed a CRISPR/Cas12a-based assay with a naked eye readout,CRISPR/Cas12a-NER.The assay can detect as few as 10 copies of the virus gene in 45 min without a special instrument and has good consistency with the qPCR assay,providing a simple and reliable on-site diagnostic method suitable for a local hospital or community testing.新型冠状病毒肺炎(COVID-19)疫情在世界范围爆发,严重危害人类健康,并对公共卫生安全造成重大影响.致病病原体SARS-CoV-2的早期快速准确检测,对及时的隔离防护、诊断治疗、有效控制疫情至关重要.为提供快速便捷的COVID-19诊断方法,本文开发了一种基于CRISPR/Cas12a的可视荧光法检测方案,命名为CRISPR/Cas12a-NER.CRISPR/Cas12a-NER无需特殊检测设备,能够在45分钟内获得病例样本的检测结果;并能有效检测到10个病毒拷贝模板数,检测结果与传统qPCR检测结果有很好的一致性.因此, CRISPR/Cas12a-NER提供了一种适用于社区医院或现场检测简单可靠的诊断方法.

关 键 词：公共卫生安全 控制疫情 隔离防护 灵敏检测 荧光法 致病病原体 社区医院 诊断方法 

分 类 号：R563.1[医药卫生—呼吸系统]