桔梗的组织培养及抗盐碱性研究  

The study on tissue culture and saline-alkali resistance of platycodon grandiflorum

作　　者：王士杰 李晨光 何颖 冯淑月 WANG Shi-jie;LI Chen-guang;HE Ying;FENG Shu-yue(College of Chinese Medicinal Materials,Jilin Agricultural Science and Technology University,Jilin 132109)

机构地区：[1]吉林农业科技学院中药学院,吉林吉林132109

出　　处：《人参研究》2020年第4期36-39,共4页Ginseng Research

基　　金：吉林省科技厅项目,项目编号:20180201005YY;省级大学生创新项目,项目编号:2019012。

摘　　要：为进一步研究桔梗组织培养条件,初步进行抗盐碱能力筛选,本实验采用白花和紫花桔梗发芽种子为外植体进行愈伤组织培养,筛选出适宜培养基及激素浓度,添加氯化钠、硫酸钠、碳酸氢钠、碳酸钠形成盐碱胁迫条件,统计组培苗的生长情况,检测组培苗中脯氨酸的含量。结果表明,白花和紫花桔梗在1/2MS培养基上的发芽率、诱导愈伤率较高;盐胁迫条件下,白花和紫花桔梗生长势与盐浓度呈现负相关,脯氨酸含量与盐浓度呈现正相关。利用组培技术能够简便、快速、准确的筛选抗盐桔梗新品种,为更好地开展育种工作提供一定理论基础。The study was on pplication of tissue culture in white and purple flower of platycodon grandiflorum to screen suitable growth medium,hormones concentrations of cultivate callus.Adding sodium chloride,sodium sulfate,sodium bicarbonate,and sodium carbonate in medium for stress conditions,we determined growth rae and the proline content of somaclone.The results showed that germination rate of platycodon grandiflorum in 1/2 MS was better,and the callus rate was higher.The growth of decreased with the increase of the salt concentration,and proline content increased with increased of the salt concentration.Tissue culture technology can be used to cultivate a new variety of salt-resistant platycodon grandiflorum,and this study provided theoretical basis for breeding work.

关 键 词：桔梗 组织培养 盐胁迫 脯氨酸含量 

分 类 号：S567.239[农业科学—中草药栽培]