Sprouty1、2、4在寻常型银屑病患者皮损组织中的表达及临床意义  

Expression of Sprouty1,2 and 4 in skin lesions of patients with psoriasis vulgaris and its significance

作　　者：李锦意 张婷 柳莉丹 陈永锋 Li Jinyi;Zhang Ting;Liu Lidan;Chen Yongfeng(Department of Dermatology,Dermatology Hospital of Southern Medical University,Guangzhou 510000,China)

机构地区：[1]南方医科大学皮肤病医院皮肤科,广州510000

出　　处：《中华皮肤科杂志》2020年第8期629-633,共5页Chinese Journal of Dermatology

基　　金：广东省医学科学技术研究基金(A2017113);广东省自然科学基金(2019A1515011202)。

摘　　要：目的研究Sprouty在寻常型银屑病患者皮损组织中的表达及其与病情严重度的相关性。方法2018年5-11月于南方医科大学皮肤病医院收集15例寻常型银屑病皮损及10例正常对照皮肤标本,采用免疫组化检测银屑病皮损与对照皮肤组织中Sprouty1、2和4蛋白的分布,Western印迹检测Sprouty1、2和4蛋白的表达,逆转录PCR(RT-PCR)检测Sprouty1、2和4 mRNA的表达。采用Pearson相关分析检验Sprouty蛋白表达量与银屑病皮损面积和严重程度指数(PASI)相关性。结果免疫组化显示,银屑病皮损和对照皮肤均有Sprouty1、2和4表达,Sprouty1在对照皮肤颗粒层细胞的细胞膜和细胞质呈强表达,在银屑病皮损颗粒层的细胞膜少量表达,Sprouty4在银屑病组中的表达强于对照组,Sprouty2在两组中均少量表达。RT-PCR和Western印迹显示,银屑病组和对照组皮肤中均有Sprouty1 mRNA和蛋白的表达,银屑病组Sprouty1 mRNA(0.844±0.169)和蛋白(0.148±0.141)的表达均明显低于对照组(分别为0.972±0.105和0.413±0.108),两组比较,均P<0.05;且银屑病组Sprouty1蛋白表达量与PASI评分呈负相关(r=-0.628,P=0.012)。银屑病组Sprouty4蛋白和mRNA表达量分别为1.306±0.283、1.727±1.017,均明显高于对照组(0.584±0.304和0.714±0.615),差异均有统计意义(t值分别为6.063和2.814,均P<0.05),且银屑病组Sprouty4蛋白表达量与PASI评分呈正相关(r=0.812,P<0.001);两组均少量表达Sprouty2蛋白和mRNA,组间差异无统计学意义(均P>0.05),且蛋白表达量与PASI评分无显著相关性(r=0.436,P=0.104)。结论银屑病皮损中Sprouty1、4蛋白表达水平与PASI评分存在相关性,提示二者与银屑病的发病相关。Objective To determine the expression of Sprouty in skin lesions of patients with psoriasis vulgaris,and to investigate its correlation with the severity of psoriasis.Methods From May 2018 to November 2018,15 lesional skin samples and 10 normal skin samples were collected from patients with psoriasis vulgaris and healthy controls respectively in Dermatology Hospital of Southern Medical University.Immunohistochemical study was performed to determine the distribution of Sprouty1,2 and 4 proteins in psoriatic lesions and normal skin tissues,Western blot analysis to determine the protein expression of Sprouty1,2 and 4,reverse transcription(RT)-PCR to measure the mRNA expression of Sprouty1,2 and 4.Pearson correlation analysis was used to analyze the correlation between Sprouty protein expression and psoriasis area and severity index(PASI)scores.Results Immunohistochemical study showed that Sprouty1,2 and 4 were all expressed in psoriatic lesions and normal skin tissues.Sprouty1 was strongly expressed in the cell membrane and cytoplasm of granular layer cells in the control group,but slightly expressed in the cell membrane of granular layer cells in the psoriasis group.Sprouty4 was more strongly expressed in the psoriasis group than in the control group,and Sprouty2 was slightly expressed in both the 2 groups.RT-PCR and Western blot analysis showed that Sprouty1 mRNA and protein were both expressed in the psoriasis group and control group,and significantly lower in the psoriasis group(0.844±0.169,0.148±0.141 respectively)than in the control group(0.972±0.105,0.413±0.108 respectively,both P<0.05);the Sprouty1 protein expression was negatively correlated with the PASI score in the psoriasis group(r=-0.628,P=0.012).The psoriasis group showed significantly increased protein and mRNA expression of Sprouty4(1.306±0.283,1.727±1.017 respectively)compared with the control group(0.584±0.304,0.714±0.615,t=6.063,2.814,respectively,both P<0.05),and the Sprouty4 protein expression was positively correlated with the PASI sco

关 键 词：银屑病 细胞外信号调节MAP激酶类 SPROUTY 疾病严重度 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]