微生物异源合成咖啡酸及其酯类衍生物研究进展  

Advances in Production of Caffeic Acid and Its Ester Derivatives in Heterologous Microbes

作　　者：王震[1] 李霞[1] 元英进[1] WANG Zhen;LI Xia;YUAN Ying-jin(Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering,Ministry of Education,Tianjin University,Tianjin 300072,China)

机构地区：[1]天津大学化工学院,教育部合成生物学前沿科学中心,系统生物工程教育部重点实验室,天津300072

出　　处：《中国生物工程杂志》2020年第7期91-99,共9页China Biotechnology

基　　金：国家自然科学基金(81502976)资助项目。

摘　　要：咖啡酸及其酯类衍生物如绿原酸、迷迭香酸和咖啡酸苯乙酯等具有天然抗氧化、抗肿瘤、抗病毒和抗炎等重要的药理活性,具有广阔的药用开发前景。从天然药物中提取或者化学合成咖啡酸及其酯类衍生物,存在含量低、提取效率不高、催化成本高昂以及环境污染等问题。随着咖啡酸及其酯类衍生物合成途径解析和合成生物学的快速发展,微生物异源合成咖啡酸及其酯类衍生物的研究已逐渐展开。对微生物异源合成咖啡酸及其酯类衍生物合成途径的最新进展以及代谢工程策略进行了综述,并讨论了目前存在的问题和未来的发展趋势。Caffeic acid and its ester derivatives like chlorogenic acid,rosmarinic acid,and caffeic acid phenethyl ester have important pharmacological activities such as natural antioxidant,antitumor,antiviral,and anti-inflammatory,and have broad prospects for medical development.Traditional extraction and chemical synthesis of caffeic acid and its derivatives from plants exist some problems such as low content,low extraction efficiency,high catalytic cost,and environmental pollution.In recent years,the study of heterologous synthesis of caffeic acid and its ester derivatives by microbes has been gradually carried out with the rapid development of synthetic biology.The recent advances in the biosynthetic pathway elucidations of caffeic acid and its ester derivatives and metabolic engineering strategies in heterologous microbes were summarized,and the current status as well as future perspectives were discussed.

关 键 词：咖啡酸 咖啡酸酯类衍生物 合成生物学 

分 类 号：Q815[生物学—生物工程]