微波消解-电感耦合等离子体质谱法测定藏药唐古特红景天中27种元素  

Microwave Digestion-ICP-MS for the Determination of 27 Elements in Traditional Tibetan Medicine Rhodiola tangutica

作　　者：续艳丽 朱仁愿 姬良亮 杨红霞[2] 杜玉枝[2] 赵磊[3] 王冠杰[4] Xu Yanli;Zhu Renyuan;Ji Liangliang;Yang Hongxia;Du Yuzhi;Zhao Lei;Wang Guanjie(Lanzhou Institute for Food and Drug Contorl,Lanzhou 730050,China;Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China;Gansu University of Chinese Medicine,Lanzhou 730000,China;National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]兰州市食品药品检验所,兰州730050 [2]中国科学院西北高原生物研究所青海省藏药药理学和安全性评价研究重点实验室,西宁810008 [3]甘肃中医药大学,兰州730000 [4]中国食品药品检定研究院,北京100050

出　　处：《中国药事》2020年第7期812-819,共8页Chinese Pharmaceutical Affairs

基　　金：青海省重点实验室专项(编号2017-ZJ-Y08);青海省藏药药理学和安全性评价研究重点实验室开放课题(编号2018-ZY-03)。

摘　　要：目的:建立微波消解-电感耦合等离子体质谱技术(ICP-MS)同时测定藏药唐古特红景天中Li、Be、Na、Mg、Al、K、Ca、Cr、Mn、Fe、Co、Ni、Cu、Zn、Ga、As、Se、Rb、Sr、Ag、Cd、Cs、Ba、Pb、Tl、U、V 27种元素的方法。方法:唐古特红景天样品经微波消解后采用ICP-MS法测定,射频功率为1.3 kW,采样深度为8.0 mm,等离子体气流速为15.0 L·min^-1,载气体积流量为1.17 L·min^-1,碰撞气体流量为5.0 mL·min^-1,蠕动泵转数为30 r·min^-1,并通过加入Bi、Ce、In、Lu、Rh、Sc、Tb混合内标对照溶液消除基体干扰。结果:27种元素在0~200μg·L^-1的线性范围内线性良好(r>0.998),定量限为0.0022~34.78μg·L^-1,检测限为0.0007~9.4μg·L^-1;精密度、稳定性、重复性试验的RSD(n=6)均小于6%;各元素平均加样回收率为73.4%~128.3%,RSD(n=6)为1.1%~9.8%。结论:该方法操作简单、灵敏度高,适用于唐古特红景天样品中多元素的同时测定。Objective:To establish a method for the determination of 27 elements including Li,Be,Na,Mg,Al,K,Ca,Cr,Mn,Fe,Co,Ni,Cu,Zn,Ga,As,Se,Rb,Sr,Ag,Cd,Cs,Ba,Pb,Tl,U and V in Rhodiola tangutica.Methods:The samples were digested via microwave and measured by inductively coupled plasma mass spectrometry(ICP-MS).Radio frequency power was 1.3 kW.Sampling depth was 8.0 mm,and the plasma gas flow rate was 15.0 L·min^-1.Carrier gas flow rate was 1.17 L·min^-1,and collision gas flow rate was 5.0 mL·min^-1.Peristaltic pump revolutions was 30 r·min^-1.The matrix effect and the mass spectrum interferences were corrected by using the internal standards of Bi,Ce,In,Lu,Rh,Sc and Tb.Results:The linear relationship of 27 elements was good in the linear range of 0-200μg·L^-1(r>0.998).The quantitative limit was 0.0022-34.78μg·L^-1,and the detection limit was 0.0007-9.4μg·L^-1.RSD values of precision,stability and repeatability were less than 6%,and the average recovery was 73.4%-128.3%with RSD of 1.1%-9.8%.Conclusion:The established method was simple and sensitive,and was suitable for the determination of multi-elements in Traditional Tibetan Medicine Rhodiola tangutica.

关 键 词：唐古特红景天 微波消解 ICP-MS 元素 

分 类 号：R95[医药卫生—药学]