miR-144-3p靶向调控肿瘤蛋白D52表达对肝癌细胞恶性生物学行为的影响  

Effect of miR-144-3p targeting on the expression of tumor protein D52 on the malignant biological behavior of hepatoma cells

作　　者：符娟[1] 孙启刚[1] 邓伟[1] 林锋[1] 吴彪[1] FU Juan;SUN Qi-gang;DENG Wei;LIN Feng;WU Biao(Department of Iinfection,Hainan Provincial People’s Hospital,Haikou 570311,Hainan Province,China)

机构地区：[1]海南省人民医院感染科,海南海口570311

出　　处：《中国临床药理学杂志》2020年第14期2070-2072,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究miR-144-3p对肝癌细胞恶性生物学行为的影响。方法实验分正常组、实验组和阴性对照组,实验组HepG2细胞转染miR-144-3p inhibitor,对照组细胞转染miR-144-3p inhibitor NC,正常组仅进行细胞培养未进行任何处理。以实时荧光定量聚合酶链反应(Real-time PCR)检测miR-144-3p表达;以CCK-8法检测HepG2细胞活率;以Transwell小室实验及Wound Healing法检测HepG2细胞侵袭、迁移能力;以双荧光素酶实验检测肿瘤蛋白D52(TPD52)表达情况。结果正常组、实验组和对照组HepG2细胞中miR-144-3p的表达量分别为1.01±0.23,3.46±0.65,0.98±0.25;干预72 h后,正常组、实验组和对照组HepG2细胞活率分别为(99.72±0.26)%,(65.86±7.73)%,(98.63±0.22)%,细胞划痕愈合率分别为(74.15±6.65)%,(50.46±4.35)%,(72.15±5.69)%,细胞穿膜细胞数分别为(78.74±4.79),(60.33±4.54),(74.05±3.21)个,TPD52 mRNA相对表达量为1.23±0.11,0.26±0.05,1.14±0.13;实验组分别与正常组和对照组比较,差异均有统计学意义(均P<0.05)。结论肝癌细胞株中miR-144-3p为低表达;miR-144-3p可以抑制肝癌细胞增殖,抑制其迁移及侵袭,其机制可能与下调TPD52基因转录表达有关。Objective To investigate the effect of miR-144-3p on the malignant biological behavior of hepatoma cells.Methods The experiment was divided into 3 groups:normal group,test group and control group.HepG2 cells in test group were transfected with miR-144-3p inhibitor,while those in control group were transfected with miR-144-3p inhibitor NC.Normal group was only cultured without any treatment.Real-time polymerase chain reaction(Real-time PCR)was used to detect the expression of miR-144-3p;CCK-8 was used to detect the cell viability of HepG2 cells;Transwell chamber test and Wound Healing methods were used to detect the invasion and migration ability of HepG2 cells;double luciferase assay was used to detect the expression of tumor protein D 52(TPD 52).Results The expression of miR-144-3p in normal group,test group and control group were 1.01±0.23,3.46±0.65 and 0.98±0.25.After 72 h of intervention,the cell viability of HepG2 in normal group,test group and control group were(99.72±0.26)%,(65.86±7.73)%,(98.63±0.22)%,cell scratch healing rates were(74.15±6.65)%,(50.46±4.35)%,(72.15±5.35)%,the number of cell penetrating membrane were 78.74±4.79,60.33±4.54,74.05±3.21,the relative expression of TPD 52 mRNA were 1.23±0.11,0.26±0.05,1.14±0.13,all with significant difference(all P<0.05).Conclusion The expression of miR-144-3p is low in hepatoma cell lines,and miR-144-3p can inhibit the proliferation,migration and invasion of hepatoma cells,which may be related to the down-regulation of TPD 52 gene transcription.

关 键 词：肝癌 HEPG2细胞 增殖 迁移 侵袭 肿瘤蛋白D52 

分 类 号：R979.1[医药卫生—药品]