Caspase抑制剂对特应性皮炎患者外周血CD4+T细胞亚群分泌细胞因子的影响  

Effect of Z-VAD-FMK,a broad caspase inhibitor,on the expression of cytokines released by CD4+T cell subset in the patients with atopic dermatitis

作　　者：李圆圆 郝宝珍 孟云芳 LI Yuanyuan;HAO Baozhen;MENG Yunfang(Department of Dermatology,Shandong Provincial Hospital Affiliated to Shandong First Medical University,Jinan 250021,China;Shandong Provincial Maternity&Child-care Hospital,Jinan 250014,China)

机构地区：[1]山东第一医科大学附属省立医院皮肤科,山东济南250021 [2]山东省妇幼保健院,山东济南250014

出　　处：《中国麻风皮肤病杂志》2020年第9期533-536,共4页China Journal of Leprosy and Skin Diseases

基　　金：山东省医药卫生科技发展计划(编号:2013WS0101);山东省重点研发计划(编号:2018GSF118177)。

摘　　要：目的:明确广谱半胱氨酸天冬氨酸蛋白酶(caspase)抑制剂对特应性皮炎(AD)患者外周血CD4+T细胞亚群分泌细胞因子的影响。方法:广谱caspase抑制剂Z-VAD-FMK与30例AD患者外周血单一核细胞(PBMCs)体外共培养后,PBMCs分为3组,分别加入Z-VAD-FMK溶液、地塞米松溶液和PBS溶液。流式细胞仪检测CD4+T细胞亚群;ELISA方法检测上清液中IFN-γ、IL-4、IL-17的浓度。结果:IFN-γ、IL-4、IL-17水平在PBS溶液组高于Z-VAD-FMK组,差异均有统计学意义(均P<0.01),在Z-VAD-FMK组与地塞米松组间比较,均无统计学意义(均P>0.05)。结论:广谱caspase抑制剂Z-VAD-FMK可抑制AD患者PBMC中CD4+T细胞Th1、Th2、Th17分泌相关细胞因子。Objective:To determine the effect of Z-VAD-FMK,a broad caspase inhibitor,on the expression of cytokines released by CD4+T cells subset in the patients with atopic dermatitis(AD).Methods:PBMCs from 30 AD patients were co-cultured with Z-VAD-FMK solution in vitro,then the cells were divided into PBS group,Z-VAD-FMK group and dexamethasone group.The levels of CD4+T cells were detected by flow cytometry.The level of IFN-γ,IL-4 and IL-17 were detected by ELISA.Results:The levels of IFN-γ,IL-4 and IL-17 in the PBS group were higher than that in the Z-VAD-FMK group(P s<0.01)and there was no significant differences of these between the Z-VAD-FMK group and dexamethasone group(P s>0.05).Conclusion:Z-VAD-FMK can inhibit the expression of cytokines released by CD4+T cells subset of Th1,Th2 and Th17 in patients with AD.

关 键 词：皮炎 特应性 半胱氨酸天冬氨酸蛋白酶 CD4+T细胞 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]