尘肺并发肺结核患者外周血CD4^+、CD8^+T细胞计数和ADA水平改变及临床意义  

Clinical significance and changes of CD4^+ and CD8^+ T cell count and ADA levels in peripheral blood of pneumoconiosis patients with pulmonary tuberculosis

作　　者：王维 何杰 周取 刘文彬 WANG Wei;HE Jie;ZHOU Qu;LIU Wenbin(Experimental Medical Center,the Sixth People′s Hospital of Chongqing,Chongqing 400060,China;School of Computer Engineering,Chongqing College Humanities,Science and Technology,Chongqing 401524,China;Department of Hepatobiliary and Pancreatic Oncology,Chongqing University Cancer Hospital,Chongqing 400030,China)

机构地区：[1]重庆市第六人民医院实验医学中心,重庆400060 [2]重庆市人文科技学院计算机工程学院,重庆401524 [3]重庆大学附属肿瘤医院肝胆胰肿瘤科,重庆400030

出　　处：《检验医学与临床》2020年第16期2299-2302,共4页Laboratory Medicine and Clinic

基　　金：重庆市南岸区决策咨询与管理创新研究计划项目(2019NAKJ06)。

摘　　要：目的探讨尘肺并发肺结核患者外周血CD4^+、CD8^+T细胞计数和血清腺苷脱氢酶(ADA)水平变化及其临床意义。方法选取2017年1月至2019年11月重庆市第六人民医院门诊及住院54例尘肺患者(尘肺组)和尘肺并发肺结核患者34例(尘肺并发肺结核组)作为研究对象,另选取该院体检健康者33例作为对照组。采用全自动细胞可视化检测系统测定尘肺组和尘肺并发肺结核组CD4^+T细胞计数、CD8^+T细胞计数,同时采用速率法检测各组血清ADA水平,并分析各组T细胞计数与ADA水平相关性。结果尘肺组和尘肺并发肺结核组CD4^+T细胞计数均低于对照组,差异有统计学意义(P<0.05)。尘肺组CD8^+T细胞计数高于尘肺并发肺结核组,差异有统计学意义(P<0.05)。尘肺并发肺结核组CD4^+/CD8^+T细胞比值均低于对照组和尘肺组,差异有统计学意义(P<0.05)。对照组、尘肺组和尘肺并发肺结核组血清ADA水平分别为(11.47±3.97)U/L、(13.01±3.28)U/L和(20.79±4.78)U/L,尘肺并发肺结核组血清ADA水平均高于对照组和尘肺组,差异有统计学意义(P<0.05)。尘肺组中CD4^+T细胞计数、CD8^+T细胞计数与血清ADA水平呈正相关(r=0.11、0.17,P=0.43、0.23);尘肺并发肺结核组CD4^+T细胞计数、CD8^+T细胞计数与血清ADA水平呈负相关(r=-0.36、-0.31,P=0.03、0.07)。结论外周血CD4^+T细胞计数、CD8^+T细胞计数和血清ADA水平对于提示尘肺患者发生肺结核感染具有一定临床意义。Objective To investigate the changes of CD4^+and CD8^+T cell count and serum adenosine dehydrogenase(ADA)levels in pneumoconiosis patients with pulmonary tuberculosis and their clinical significance.Methods From January 2017 to November 2019,54 outpatient and inpatient patients with pneumoconiosis(pneumoconiosis group)and 34 patients with pneumoconiosis complicated with pulmonary tuberculosis(pneumoconiosis complicated with pulmonary tuberculosis group)in Chongqing Sixth People′s Hospital were selected as the research objects,and 33 healthy patients in a hospital were selected as the control group.CD4^+T cell count and CD8^+T cell count in pneumoconiosis group and pneumoconiosis complicated with pulmonary tuberculosis group were measured by automatic cell visualization detection system.Meanwhile,serum ADA level in each group was detected by rate method,and the correlation between T cell count and ADA level in each group was analyzed.Results CD4^+T cell count in both pneumoconiosis group and pneumoconiosis complicated with pulmonary tuberculosis group were lower than those in control group,the difference was statistically significant(P<0.05).The number of CD8^+T cell count in the pneumoconiosis group was higher than that in the pneumoconiosis group with pulmonary tuberculosis,the difference was statistically significant(P<0.05).The CD4^+/CD8^+T cell ratio of pneumoconiosis complicated with pulmonary tuberculosis group was lower than that of the control group and pneumoconiosis group,the difference was statistically significant(P<0.05).The serum ADA levels of the control group,the pneumoconiosis group,and the pneumoconiosis complicated with pulmonary tuberculosis group were(11.47±3.97)U/L,(13.01±3.28)U/L and(20.79±4.78)U/L,respectively.The serum ADA levels of the pneumoconiosis complicated with pulmonary tuberculosis group were higher than those of the control group and the pneumoconiosis group,with statistically significant differences(P<0.05).CD4^+T cell count and CD8^+T cell count in pneumoconiosis group we

关 键 词：尘肺 肺结核 CD4^+T细胞计数 CD8^+T细胞计数 腺苷脱氢酶 

分 类 号：R135.2[医药卫生—劳动卫生]