基于核酸适配体检测平台的设计模式  

Design modes for aptamer-based detection platforms

作　　者：刘小慧 彭虎[1,2] 张艳琼 Liu Xiaohui;Peng Hu;Zhang Yanqiong(Medical College,China Three Gorges University,Yichang 443002,China;Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy,China Three Gorges University,Yichang 443002,China)

机构地区：[1]三峡大学医学院,宜昌443002 [2]三峡大学肿瘤微环境与免疫湖北省重点实验室,宜昌443002

出　　处：《解剖学杂志》2020年第4期339-342,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金(81670555);肿瘤微环境与免疫湖北省重点实验室开放基金(2019KZL05);三峡大学研究生科技创新基金(2018SSPY106)。

摘　　要：核酸适配体可用作信号识别元件,具有高亲和力、高特异性、高稳定性和易被修饰的特性。核酸适配体的检测平台包括核酸适配体探针和核酸适配体传感器,可精确、实时地检测生物分子,对临床疾病相关生物标志物的检测有较大的应用潜力。现将基于核酸适配体检测平台的设计模式作一综述,讨论其研究现状、局限性和未来发展方向。As a newly-emerging field,aptamer can be used as a signal recognition element.It has high affinity,high specificity,stronger stability and is easy to be modified.The aptamer-based detection platform includes aptamer probe and aptasensor,which can detect biomolecules accurately and in real time.Therefore,the aptamer-based detection platform has great potential in the detection of biomarkers related diseases in clinic.This review summarized the application of various models according to their characteristics,and discussed the current limitations and directions in future.

关 键 词：核酸适配体 核酸适配体探针 核酸适配体传感器 设计模式 

分 类 号：R440[医药卫生—诊断学] TP212[医药卫生—临床医学]