离子束诱变选育高效降解有机磷农药菌株  

Breeding of a highly effective organophosphorus-degrading mutant bacteria obtained by ion beam irradiation

作　　者：彭轶楠 巩晓芳 祝英 季彬 陆栋[2] 杨晖 王治业 PENG Yinan;GONG Xiaofang;ZHU Ying;JI Bin;LU Dong;YANG Hui;WANG Zhiye(Key Laboratory of Microbial Resources Exploitation and Application of Gansu Province,Institute of Biology,Gansu Academy of Sciences,Lanzhou 730000,China;Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,China)

机构地区：[1]甘肃科学院生物研究所甘肃省微生物资源开发利用重点实验室,兰州730000 [2]中国科学院近代物理研究所,兰州730000

出　　处：《辐射研究与辐射工艺学报》2020年第4期57-66,共10页Journal of Radiation Research and Radiation Processing

基　　金：甘肃省科技计划项目(18JR3RA253);甘肃省科学院创新团队项目(2019CX004-01);甘肃省科学院应用研发项目(2017JK-11);甘肃省科学院优秀青年科技创新基金项目(2019QN-02)资助。

摘　　要：采用12C6^+离子束辐照诱变有机磷降解菌,结合选择性培养基初筛突变菌株,摇瓶复筛获得1株高效降解有机磷菌株LA-1,并对有机磷农药降解进行研究。结果表明:吸收剂量为150 Gy时诱变效果最佳,通过筛选和连续8代培养,获得稳定的突变菌株LA-1,其降解有机磷量为161.71 mg/L,较原始菌株解磷能力提高了2.89倍,当有机磷农药辛硫磷和氧乐果浓度分别为800μg/mL和400μg/mL时,菌株的降解率分别为93.5%和83.6%。突变菌株LA-1对有机磷农药具有良好的降解效果。An organophosphorus-degrading Bacillus subtilis strain was irradiated by 12C6+ion beam and a highly efficient organophosphorus-degrading LA-1 strain was selected through selective breeding medium and shake flask experiments.The effect of organophosphorous pesticide degradation was also studied.The results showed that the highly stable LA-1 mutant strain was screened through eight generation propagation at an absorbed dose of 150 Gy.The amount of degraded organophosphorus was 161.71 mg/L,which was 2.89 times higher than that of the original strain.The degradation rates were 93.5%on phoxin and 83.6%on omethoate when the concentration reached 800μg/mL and 400μg/mL,respectively.The LA-1 mutant strain has a promising organophosphorus pesticide degradation rate.

关 键 词：离子束辐照 诱变育种 有机磷农药降解 枯草芽孢杆菌 

分 类 号：Q691[生物学—生物物理学] TL99[核科学技术—核技术及应用]