普氏野马两种肠道寄生虫ITS序列的扩增与分析  

Amplification and Analysis of ITS Sequence of Intestinal Parasites in Przewalski's Wild Horse(Equus ferus ssp.przewalskii)

作　　者：李乔 赵建友[3] 杜晓华[2] 卢伟[3] 张春林 董建英 温永强 周应杰[3] 刘霞[1] LI Qiao;ZHAO Jian-You;DU Xiao-Hua;LU Wei;ZHANG Chun-Lin;DONG Jian-Ying;WEN Yong-Qiang;ZHOU Ying-Jie;LIU Xia(College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;State Forest Administration Gansu Protection Centre of Threatened Species,Wuwei 733000,China)

机构地区：[1]甘肃农业大学生命科学技术学院,兰州730070 [2]甘肃农业大学动物医学院,兰州730070 [3]国家林业局甘肃濒危动物保护中心,武威733000

出　　处：《农业生物技术学报》2020年第7期1277-1286,共10页Journal of Agricultural Biotechnology

基　　金：甘肃省科技厅重点研发科技项目(17YF1NH084);甘肃农业大学校企横向合作项目(032-031119;032-031120;032-031121)

摘　　要：普氏野马(Equus ferus ssp.przewalskii)作为一种濒危保护动物,其健康成长和种群数量的扩大对种质资源保护具有特殊价值,其体内寄生虫可对机体产生不同程度的病理损害。本研究拟鉴定国家林业局甘肃濒危动物保护中心普氏野马肠道内寄生虫的种类,首先分离普氏野马群体驱虫后粪便中的虫体,进行形态学特征初步鉴定,之后运用生物信息学方法对扩增虫体的内转录间隔区(internal transcribed spacer,ITS)序列进行比对。结果显示,普氏野马肠道共分离出2种虫体,形态学鉴定初步推断为马副蛔虫(Parascaris equorum)和杯环属线虫(Cylicocyclus sp.),分别命名为PE和CN;PE虫体ITS区全长865 bp(GenBank No.MT579850),其中ITS-1、5.8S rDNA和ITS-2的长度分别为421、156和288 bp,其与马副蛔虫的同源性为99.88%;CN虫体ITS区全长843 bp(GenBank No.MT581488),其中ITS-1、5.8S rDNA和ITS-2的长度分别为370、153和320 bp,与鼻状杯环线虫(Cylicocyclus nassatus)的核酸序列同源性为99.41%。上述结果表明,普氏野马体内获取的PE虫体为马副蛔虫、CN虫体为鼻状杯环线虫。本研究结果可为普氏野马的健康圈养提供参考依据。Przewalski's wild horse(Equus ferus ssp.przewalskii)is a subspecies of E.ferus and is considered as endangered animals.The healthy growth and continuous expansion in their population have special value for germplasm resource protection.The parasites in Przewalski's wild horse can produce different degrees of pathological damage to the organism.The present study aimed to identify the intestinal parasite species in Przewalski's wild horses which were taken from the State Forest Administration Gansu Protection Centre of Threatened Species.During sampling procedures,the worms were separated in the faeces of Przewalski's wild horses.Morphological characteristics were identified and ITS(internal transcribed spacer)sequences were compared using bioinformatic methods.The results showed that 2 kinds of worms named PE and CN were isolated from intestinal tracts of Przewalski's wild horses,and preliminarily identified as Parascaris equorum and Cylicocyclus sp.by morphology observation.The full length of PE ITS region was 865 bp(GenBank No.MT579850),and the length of ITS-1,5.8S rDNA and ITS-2 was 421,156 and 288 bp,respectively;and the homology with Parascaris equorum was 99.88%.The full length of CN ITS region was 843 bp(GenBank No.MT581488),and the length of ITS-1,5.8S rDNA and ITS-2 was 370,153 and 320 bp,respectively;and the homology with C.nassatus was 99.41%.The above results showed that PE sample collected from Przewalski's wild horses was Parascaris equorum and the CN sample was C.nassatus.The results of this study could provide a reference for healthy breeding of Przewalski's wild horses.

关 键 词：普氏野马 寄生虫 内转录间隔区(ITS) 同源性分析 

分 类 号：S852.71[农业科学—基础兽医学]