益肾泄浊法对糖尿病肾病大鼠Col Ⅳ、FN蛋白及基因表达的影响  

Effect of kidney-tonifying and turbidity-discharging method on Col IV,FN protein and gene expression in diabetic nephropathy rats

作　　者：邵燕燕 郑晓静 薛化成 梁祎 甘旗旗 焦畔畔 SHAO Yanyan;ZHENG Xiaojing;XUE Huacheng;LIANG Yi;GAN Qiqi;JIAO Panpan(Department of Nephrology,Xi'an Traditional Chinese Medicine Hospital,Xi'an 710021,China)

机构地区：[1]西安市中医医院肾病科,陕西西安710021

出　　处：《广东药科大学学报》2020年第4期509-513,共5页Journal of Guangdong Pharmaceutical University

基　　金：2019西安市级中医药科研项目(SZJ201908);西安市名中医工作室建设项目(邵燕燕工作室,编号:201716)。

摘　　要：目的探讨益肾泄浊法对糖尿病肾病大鼠的相关生化指标以及肾脏的组织病理改变的生物学效应,并探讨益肾泄浊法对糖尿病肾病大鼠胶原蛋白Ⅳ(Collagen Ⅳ)、纤维粘连蛋白(FN)及基因表达的影响。方法采用链脲佐菌素(STZ)建立大鼠糖尿病(diabetes mellitus,DM)模型并给予不同剂量益肾泄浊法组方的中药干预,进行生化检查、光镜检查、蛋白免疫印迹试验(Western blot)和实时荧光定量聚合酶链式反应(Real-time Polymerase Chain Reaction)。结果与模型组比较,益肾泄浊法组可以降低血肌酐、血尿素氮、24 h尿白蛋白和糖化血红蛋白;通过组织切片观察,益肾泄浊法组可以降低大鼠肾脏糖原、粘液蛋白的产生;与模型组相比,益肾泄浊法高、中、低剂量组均能使Col Ⅳ、FN蛋白和mRNA的表达量减少。结论益肾泄浊法能使糖尿病肾病大鼠生化指标发生改变,减少Col Ⅳ、FN蛋白及基因表达。Objective To explore the biological effects of kidney-tonifying and turbidity-discharging method on biochemical index, renal histopathological change and collagen Ⅳ( Col Ⅳ) and fibronectin( FN)expression in rats with diabetic nephropathy. Methods The rat DM model was established by streptozotocin( STZ) and treated with different doses of kidney-tonifying and turbidity-discharging method. Biochemical examination,light microscopy,western blot and real-time PCR were performed. Results Compared with the model group, kidney-tonifying and turbidity-discharging method reduced serum creatinine, blood urea nitrogen,24 h urinary albumin and glycosylated hemoglobin. Tissue section observation showed that kidneytonifying and turbidity-discharging method decreased the production of glycogen and mucus protein in rat kidney. Meantime,the different dose groups of kidney-tonifying and turbidity-discharging method reduced the expression of Col Ⅳ,FN protein and mRNA. Conclusion Kidney-tonifying and turbidity-discharging method can change the biochemical indexes and reduce the expression of Col Ⅳ and FN.

关 键 词：益肾泄浊法 糖尿病肾病 胶原蛋白Ⅳ(CollagenⅣ) 纤维粘连蛋白(FN) 

分 类 号：R285.5[医药卫生—中药学]