烟草低温早花相关基因NtDUF599的表达及功能分析  

Expression and functional analysis of NtDUF599 related to chilling-induced early flowering in tobacco(Nicotiana tabacum L.)

作　　者：徐国云[1] 翟妞[1] 许亚龙[1] 谢小东[1] 张慧[1] 郑庆霞[1] 张剑锋[1] 刘萍萍[1] 陈千思[1] 金立锋[1] 周会娜[1] XU Guoyun;ZHAI Niu;XU Yalong;XIE Xiaodong;ZHANG Hui;ZHENG Qingxia;ZHANG Jianfeng;LIU Pingping;CHEN Qiansi;JIN Lifeng;ZHOU Huina(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China)

机构地区：[1]中国烟草总公司郑州烟草研究院,郑州450001

出　　处：《烟草科技》2020年第8期1-6,23,共7页Tobacco Science & Technology

基　　金：郑州烟草研究院院长科技发展基金项目“烟草低温早花相关基因的筛选及鉴定”(902018CA0280)。

摘　　要：为研究烟草低温诱导早花的作用机制,利用转录组分析筛选获得了烟草低温早花相关基因NtDUF599。NtDUF599基因编码一个包含236个氨基酸的未知功能蛋白,受低温诱导高表达。构建该基因的RNAi干扰(RNA interference,RNAi)载体,并通过农杆菌介导法进行烟草遗传转化,获得10个转基因株系。将转基因株系和非转基因对照株系在苗期低温(12℃)处理10 d后,在正常条件下生长至现蕾期。结果显示,转基因烟株的现蕾时间比对照提前10 d左右,且叶片数目减少。本研究首次发现DUFs(Domain of unknown functions)基因参与烟草低温诱导早花过程。To investigate the mechanism underlying early flowering in tobacco caused by chilling stress,NtDUF599,a candidate gene related to the phenomenon was screened by RNA-seq analysis.NtDUF599 encoded a protein containing 236 amino acids with unknown functions,and its expression was highly induced by chilling stress.A RNAi plasmid,pBWA(V)HS-NtDUF599-RNAi vector,was constructed and a total of 10 different transgenic lines were generated by Agrobacterium-mediated transformation.Seedlings of non-transgenic and transgenic plants were treated with chilling stress(12℃)for 10 days and kept grown until bud stage under normal growth condition.We found that the emergence of flower buds of NtDUF599-RNAi transgenic plants was approximately 10 days earlier than non-transgenic plants.Furthermore,leaf numbers of NtDUF599-RNAi transgenic plants were less than those of non-transgenic plants.Those results revealed for the first time that DUFs(Domain of unknown functions)were involved in the chilling-induced early flowering in tobacco.

关 键 词：烟草 低温胁迫 早花 RNAI DUF结构域 

分 类 号：T162.5[一般工业技术]