NNK长期暴露对小鼠毒性和Prx基因表达的影响  

Effects of long-term NNK exposure on toxicity and Prx gene expression in mice

作　　者：时桂芹 沈佳鑫 任菲 韩亚伟 SHI Guiqin;SHEN Jiaxin;REN Fei;HAN Yawei(Zhengzhou University of Light Industry,Zhengzhou 450002,China)

机构地区：[1]郑州轻工业大学,郑州市450002

出　　处：《烟草科技》2020年第8期36-43,共8页Tobacco Science & Technology

基　　金：国家自然科学基金项目“家蚕特异性扩增基因Pxts在病菌免疫应答中的功能及作用机制研究”(31802138);河南省科技攻关项目“高表达SOD对酿酒酵母细胞老化过程中生理生化的影响及其在啤酒风味改良中的应用研究”(172102110208);星空众创空间孵化项目“芍药籽壳黄酮类化合物的分离提取及其在食品工业中的应用研究”(2018ZCKJ218)。

摘　　要：为研究4-(甲基亚硝氨基)-1-(3-吡啶基)-1-丁酮(NNK)对小鼠的毒性以及与Prx(Peroxiredoxin)基因表达的关系,通过腹腔注射方式对小鼠进行NNK染毒(2.5和5.0 mg/kg),24周后利用苏木精-伊红(HE)染色和实时荧光定量聚合酶链反应(qRT-PCR)技术考察了NNK对小鼠肺、肝、肾和睾丸组织的毒性以及对Prx家族基因表达的影响。结果表明:①与对照组小鼠相比,处理组小鼠好斗性增强,进食量无较大变化,但染毒5周后,小鼠体质量降低。②处理组小鼠出现肺泡隔增生,肝血窦与肝细胞核增大,肾小球玻璃样变性、破碎,睾丸生精小管中各级精细胞明显减少。③在NNK胁迫下,Prx基因均被诱导;同一Prx基因在不同组织中的表达量不同,处理组小鼠肝组织中Prx基因的表达量最高,是对照组的34.8倍。④在对照组小鼠组织中Prx5基因表达量均较低,但NNK处理后其表达量升高,在5.0 mg/kg NNK处理后,其表达量升高更明显。因此,推测Prx5基因可能在NNK胁迫下发挥着重要的抗氧化作用。In order to study the toxicity of 4-methylnitrosamine-1-(3-pyridyl)-1-butanone(NNK)to mice and its relationship with Prx(peroxiredoxin)gene expression,experimental mice were exposed to NNK via intraperitoneal injection(2.5 and 5.0 mg/kg).After 24 weeks,HE(hematoxylin-eosin)staining and qRT-PCR(quantitative real-time polymerase chain reaction)were used to measure the toxicity of NNK on the lung,liver,kidney and testis of the treated mice and NNK’s effect on the expression of Prx family genes.The results showed that:1)Compared with the control group,the mice in the treatment group were more aggressive and had no significant change in food intake,however their body weight decreased five weeks after the exposure.2)The mice in the treatment group had hyperplasia in alveolar septum,enlargement in hepatic sinuses and nuclei,hyaline degeneration and fragmentation of glomeruli,and decrease of spermatocytes in testicular seminiferous tubules.3)Under the NNK stress,Prx genes were induced.The Prx gene expression differed in different tissues,the highest expression in the liver tissue was 34.8 times higher than that of the control mice.4)In the control group,the Prx5 gene expression was lower,while it increased after the NNK treatment,especially at 5.0 mg/kg NNK treatment.Therefore,it is suggested that Prx5 gene might play an important role in anti-oxidation action under NNK stress.

关 键 词：NNK Prx基因 基因表达 小鼠 

分 类 号：TS416[农业科学—烟草工业]