用于骨科植入体的纳米银/氧化钛纳米管复合抗菌涂层  

Silver Nanoparticles Incorporated Titania Nanotube Coating for Orthopedic Implants with Antibacterial Properties

作　　者：方思懿 方明 李翔[1] FANG Siyi;FANG Ming;LI Xiang(Inorganic Nonmetallic Materials Institute,School of Materials Science and Engineering,Zhejiang University,Hangzhou 310027,China;CANWELL Medical Co Ltd.,Jinhua 321000,China)

机构地区：[1]浙江大学材料科学与工程学院无机非金属材料研究所,浙江杭州310027 [2]浙江科惠医疗器械股份有限公司,浙江金华321000

出　　处：《材料科学与工程学报》2020年第3期363-369,408,共8页Journal of Materials Science and Engineering

基　　金：国家自然基金面上资助项目(51672247)。

摘　　要：以乙二醇含0.5wt.%氟化铵和5vol.%蒸馏水为电解液,利用阳极氧化法在钛表面制备出TiO 2纳米管。之后对制备得到的TiO 2纳米管使用聚多巴胺进行表面改性,再用浸渍法加紫外光还原法制备得到负载银纳米颗粒的TiO 2纳米管(AgNPs/TNTs)涂层材料。通过改变AgNO 3溶液的浓度可以得到不同AgNPs负载量的TiO 2纳米管。利用多种表征方法,对AgNPs/TNTs进行微结构表征;通过抗菌实验和体外细胞实验,对AgNPs/TNTs进行了抗菌性能和细胞相容性测试。研究结果表明:在70V电压下阳极氧化可得到管径约为100nm的TiO 2纳米管。AgNPs在TiO 2纳米管中的负载量随AgNO 3溶液的浓度的增大而增多。AgNPs/TNTs的Ag+释放可持续14天以上,试样抗菌性能NT-PD-0.5Ag>NT-PD-0.1Ag>NT-PD-0.02Ag。含银量较低的试样NT-PD-0.1Ag和NT-PD-0.02Ag具有相对较好的细胞相容性,而含银量较大的试样NT-PD-0.5Ag的细胞毒性较大。AgNPs/TNTs复合涂层可具有良好的抗菌性能和细胞相容性,为用于产业化生产提供参考。TiO2 nanotubes were prepared on titanium surface via anodic oxidation with ethylene glycol containing 0.5 wt.%ammonium fluoride and 5 vol.%distilled water.After surface-modification with polydopamine,TiO 2 nanotubes were incorporated with silver nanoparticles(AgNPs/TNTs)by impregnation and UV reduction.By changing the concentration of AgNO 3 solution,AgNPs/TNTs coatings with varied microstructures,characterized by some experiments,were obtained.Anti bacterial experiments and cell compatibility test were also done on the AgNPs/TNTs.TiO 2 nanotubes with diameter of^100nm were produced by anodizing at 70V.The loading of AgNPs and antibacterial properties were enhanced expectably with increased concentration of AgNO 3 solution.The Ag+release of AgNPs/TNTs can be maintained for more than 14 days.The samples with lower silver content present excellent cytocompatibility,while the sample with high silver content induced certain cytotoxicity.Overall,AgNPs/TNTs composite coatings investigated hereby have presented satisfactory antibacterial properties and cell compatibility,which may provide valuable reference for industrial production.

关 键 词：TIO2纳米管 银纳米粒子 阳极氧化 抗菌性能 

分 类 号：TB333[一般工业技术—材料科学与工程] R318.08[医药卫生—生物医学工程]