黄芪、莪术联合顺铂对人肝癌裸鼠CD147、iNOS表达的影响  

Effects on expression of CD147,iNOS in nude mice of human hepatocarcinoma transplantation model by compatibility of Radix Astragali and Curcuma and combination with cisplatin

作　　者：臧文华 胡久略 唐德才[3] 尹刚[3] 郅琳 丁生晨 ZANG Wen-hua;HU Jiu-lue;TANG De-cai;YIN Gang;ZHI Lin;DING Sheng-chen(Zhang Zhongjing College of Traditional Chinese Medicine,Nanyang Institute of Technology,Nanyang 453000,China;Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation,Nanyang 453000,China;Nanjing University of Chinese Medicine,Nanjing 210046,China)

机构地区：[1]南阳理工学院张仲景国医国药学院,河南南阳453000 [2]河南省张仲景方药与免疫调节重点实验室,河南南阳453000 [3]南京中医药大学基础医学院,江苏南京210046

出　　处：《时珍国医国药》2020年第4期785-788,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金青年基金(81503270,81503267);国家自然科学基金面上项目(81073072,81373990);河南省科技厅科技攻关项目(182102310637);河南省高等学校重点科研项目(15A360011);河南省中医药科学研究专项课题(2015ZY02004)。

摘　　要：目的探讨黄芪、莪术配伍联合顺铂(DDP)对人肝癌裸鼠CD147、iNOS表达的影响。方法构建人肝癌裸鼠原位移植瘤模型。成瘤后,将其随机分为8组:模型对照组、阳性对照组(DDP)、黄芪莪术配伍高剂量组(H)、黄芪莪术配伍中剂量组(M)、黄芪莪术配伍低剂量组(L)、黄芪莪术配伍高剂量+顺铂组(H+DDP)、黄芪莪术配伍中剂量+顺铂组(M+DDP)、黄芪莪术配伍低剂量+顺铂组(L+DDP),每组8只,给药15d,免疫组织化学染色及Real-time RT-PCR检测肿瘤组织中CD147、iNOS蛋白及mRNA的表达。结果与模型对照组相比,H、M、H+DDP、M+DDP、L+DDP组CD147、iNOS蛋白及mRNA表达均显著下降(P<0.05或P<0.01),并与剂量呈一定的正相关性。与DDP组相比,H+DDP组CD147、iNOS蛋白表达降低(P<0.05或P<0.01)。结论黄芪、莪术配伍可能是通过下调CD147、iNOS蛋白及mRNA表达对肿瘤新生血管生成产生影响。Objective To study the effects on expression of CD147, iNOS in nude mice of human hepatocarcinoma transplantation model by compatibility of Radix Astragali and Curcuma and combination with cisplatin(DDP). Methods Human hepatocarcinoma cells HepG2 were transplanted into nude mice to form the study model, and the modeled mice were divided at random into 8 groups: model group, cisplatin(DDP)group(DDP), high dosage of Radix Astragali and Curcuma(H), intermediate dosage of Radix Astragali and Curcuma(M), low dosage of Radix Astragali and Curcuma(L), high dosage and cisplatin group(H+DDP), intermediate dosage and cisplatin group(M+DDP), low dosage and cisplatin group(L+DDP),8 in each group. The expression of CD147, iNOS protein and mRNA in tumor tissues were detected by the immunohistochemical staining technique and Real-time RT-PCR. Results Compared with the model group, the expression of CD147, iNOS protein and mRNA decreased significantly(P<0.05 or P<0.01) in H, M, H+DDP, M+DDP and L+DDP group. It was positively correlated with the dose. Compared with DDP group, the expression of CD147, iNOS protein obviously decreased(P<0.05 or P<0.01) in H+DDP group. Conclusion Compatibility of radix astragali and curcuma may affect tumor angiogenesis by down-regulating the expression of CD147, iNOS protein and mRNA.

关 键 词：黄芪 莪术 肝细胞癌 CD147 INOS 

分 类 号：R285.5[医药卫生—中药学]