利用SSR标记技术鉴定西瓜杂交种纯度的研究  

Identification of Purity of Watermelon Hybrid by the SSR

作　　者：韩道杰 冯锡鸿 马长河 孙德祥 申太荣 钱瑾 董建力 Han Daojie;Feng Xihong;Ma Changhe;Sun Dexiang;Shen Tairong;Qian Jin;Dong Jianli(Ningxia Zhongqing Agricultural Science and Technology Co.,Ltd.,Yinchuan,Ningxia 750002;Agricultural Biotechnology Research Center,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750002)

机构地区：[1]宁夏中青农业科技有限公司,宁夏银川750002 [2]宁夏农林科学院农业生物技术研究中心,宁夏银川750002

出　　处：《宁夏农林科技》2020年第5期9-11,共3页Journal of Ningxia Agriculture and Forestry Science and Technology

基　　金：宁夏回族自治区科技支撑项目“西甜瓜优新品种引选及制种技术集成与示范”;宁夏回族自治区农业育种专项(NXNYYZ20150303)。

摘　　要：为快速高效鉴定西瓜杂交种纯度,利用SSR标记技术对西瓜新品种“中青5号”杂交种进行鉴定。结果表明:筛选出SSR25引物在150 bp处扩增出父、母本和杂交种共有的条带,在160 bp和180 bp处扩增出杂交种特征带。SSR25标记适用于“中青5号”杂交种纯度鉴定,SSR鉴定结果的准确性高于大田鉴定结果。研究结果为生产应用提供科学依据。To quickly and efficiently identify the purity of watermelon hybrids,the SSR was used to identify the new watermelon variety"Zhongqing No.5"hybrid.The results showed that the SSR25 primers were screened to amplify the bands shared by the parent and hybrid species at 150 bp;hybrid characteristic bands were amplified at 160 bp and 180 bp.SSR25 is suitable for purity identification of"Zhongqing No.5"hybrid line.The accuracy of the SSR identification is higher than that of field identification.The results provide scientific basis for production and application.

关 键 词：西瓜 SSR标记 杂交种 纯度鉴定 

分 类 号：S651[农业科学—果树学]