UHPLC法测定不同产地雷公藤饮片中雷公藤甲素和红素的含量  

Determination of Triptolide and Celastrol in T.wilfordii from Several Habitats by UHPLC

作　　者：葛丹丹[1] 高家荣[2] 鲍学梅[1] 杨满琴[1] 徐倩[1] 苏毅[1] GE Dan-dan;GAO Jia-rong;BAO Xue-mei;YANG Man-qin;XU Qian;SU Yi(Second Affliated Hospital of Anhui University of Chinese Medicine,Anhui Hefei 230061;First Affliated Hospital of Anhui University of Chinese Medicine,Anhui Hefei 230061;Second Affliated Hospital of Anhui University of Chinese Medicine,Anhui Hefei 230061,China)

机构地区：[1]安徽中医药大学第二附属医院,安徽合肥230061 [2]安徽中医药大学第一附属医院,安徽合肥230061

出　　处：《广州化工》2020年第16期91-93,共3页GuangZhou Chemical Industry

基　　金：安徽中医药大学2018年度校级科研项目(编号:2018zryb18)。

摘　　要：对市售不同产地的雷公藤饮片中雷公藤甲素和雷公藤红素进行含量测定。采用超高液相色谱法。色谱柱为Waters Acquity UPLC BEH C 18柱(2.1 mm×100 nm,1.7μm),流动相为乙腈-0.1%磷酸水,梯度洗脱,流速为0.5 mL/min,柱温25℃,检测波长210 nm;两种有效成分在各自的线性范围内线性关系良好,该实验所建立的方法简便,重复性好。不同产地雷公藤饮片中雷公藤甲素和雷公藤红素的含量有显著差异,表明市售雷公藤饮片的质量存在明显差别,为雷公藤饮片进一步的临床应用提供参考。Triptolide and Celastrol in T.wilfordii on marketed from different regions were determined by using ultra-performance liquid chromatography analysis.Chromatographic column was Waters Acquity UHPL BEH C 18 column(2.1 mm×100 nm,1.7 microns),mobile phase was acetonitrile-0.1%phosphoric acid water,the flow rate was 0.5 mL/min,column temperature was 25℃,and detection wavelength was set at 210 nm.The two kinds of active ingredients in their respective inside had good linear range,and established method was simple and had good repeatability.The different origin of tripterygium wilfordii slices of Triptolide and Celastrol had significant difference,which showed that the quality of commercial tripterygium wilfordii had obvious difference,all this can provide reference for the clinical application.

关 键 词：雷公藤 UHPLC 雷公藤甲素 雷公藤红素 含量测定 

分 类 号：R917[医药卫生—药物分析学]