分选循环肿瘤细胞的微流控芯片及临床应用研究  

Research on microfluidic chip for sorting circulating tumor cells and its clinical application

作　　者：王艳敏 王浩 金鎏 贾春平[2] 张森 赵建龙[2] WANG Yanmin;WANG Hao;JIN Liu;JIA Chunping;ZHANG Sen;ZHAO Jianlong(College of Life Sciences,Shanghai Normal University,Shanghai 200030,China;State Key Laboratory of Transducer Technology,Shanghai Institute of Microsystem and Information Technology,Chinese Academy of Sciences,Shanghai 200050,China)

机构地区：[1]上海师范大学生命科学学院,上海200030 [2]中国科学院上海微系统与信息技术研究所传感技术联合国家重点实验室,上海200050

出　　处：《传感器与微系统》2020年第8期9-12,共4页Transducer and Microsystem Technologies

基　　金：国家自然科学基金资助项目(81472751);中国科学院科技服务网络计划项目(KFJ-STS-ZDTP-061)。

摘　　要：循环肿瘤细胞(CTCs)是由肿瘤原发灶脱落进入外周血循环的肿瘤细胞,与肿瘤转移有着密切的关系。基于物理尺寸设计一种捕获CTCs的微流控芯片,芯片包含分流通道,捕获细胞团的六边柱阵列和单细胞过滤通道阵列,并结合细胞免疫荧光染色法,用肿瘤细胞特异性标记物CK8+18、CK19、AntiVimentin、PD-L1染色表征鉴别CTCs。芯片对细胞的捕获率可达80%以上,并在100名肺癌患者和100名正常人对照组中检测了其CTCs数量,发现肺癌患者的CTCs数目显著高于正常人(P<0.001),接受者操作特征曲线(ROC)分析显示,此微流控装置具有较高的灵敏度(92%)和特异性(91%)。总之,该微流控芯片能有效地捕获CTCs,表征它们在癌症诊断中的生物学特性,具有很好的临床应用价值。Circulating tumor cells(CTCs)are tumor cells that are detached from the primary tumor into the peripheral blood circulation and have a close relationship with tumor metastasis.A microfluidic chip for capturing CTCs is designed based on physical dimensions.The chip contains shunt channels,hexagonal column array for capturing cell clusters and single cell filtration channels.And combined with cellular immunofluorescence staining,using specific markers of tumor cell(CK8+18、CK19、Anti-Vimentin、PD-L1)to identify CTCs.The microfluidic chip has a cell capture rate of more than 80%.CTCs are detected in 100 patients with lung cancer and in 100 healthy donors,the number of CTCs in lung cancer patients is significantly higher than that in normal people(P<0.001).The receiver operating characteristic curve(ROC)analysis shows that the microfluidic device has apparent high sensitivity(92%)and specificity(91%).In conclusion,this size-based microfluidic chip can effectively capture CTCs and characterize their biological characteristics in cancer diagnosis,which has good clinical application value.

关 键 词：微流控芯片 细胞免疫荧光染色法 循环肿瘤细胞 尺寸 捕获率 灵敏性 特异性 

分 类 号：TN212.3[电子电信—物理电子学] TP212[自动化与计算机技术—检测技术与自动化装置]