Sensititre YeastOne显色药敏板与微量肉汤稀释法检测曲霉体外抗真菌药物敏感性的一致性研究  

Comparative evaluation of Sensititre YeastOne colorimetric panel with broth microdilution method for in vitro antifungal susceptibility testing of Aspergillus

作　　者：李颖[1,3] 王贺 张戈[2,3] 刘文静 徐英春[2,3] LI Ying;WANG He;ZHANG Ge;LIU Wen-jing;XU Ying-chun(Department of Clinical Laboratory,Xuanwu Hospital,Capital Medical University,Beijing 100053,China;Department of Clinical Laboratory,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences,Beijing 100730,China;Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases,Beijing 100730,China)

机构地区：[1]首都医科大学宣武医院检验科,北京100053 [2]中国医学科学院北京协和医院检验科,北京100730 [3]侵袭性真菌病机制研究与精准诊断北京市重点实验室,北京100730

出　　处：《中国真菌学杂志》2020年第4期197-201,共5页Chinese Journal of Mycology

摘　　要：目的评估显色药敏板Sensititre YeastOne与微量肉汤稀释法检测曲霉体外抗真菌药物敏感性的一致性。方法使用同样的孢子悬液按照CLSI M38-A2微量肉汤稀释法和Sensititre YeastOne YO10对307株曲霉进行体外抗真菌药物敏感性试验,计算两种药敏方法结果间的基本一致性、分类一致性以及错误率。结果CLSI M38-A2药敏试验显示98.4%(302/307)曲霉菌株对测试的6种抗真菌药物持野生型状态,Sensititre YeastOne则检出97.1%(298/307)菌株为野生型。除伊曲康唑外,其余5种抗真菌药物在Sensititre YeastOne与CLSI M38-A2间的基本一致性均>90%。两种方法在曲霉药物敏感性检测的分类一致性为98.1%(301/307),极显著错误率为0.3%(1/307),显著错误率为1.6%(5/307)。结论Sensititre YeastOne在检测曲霉体外药物敏感性方面与CLSI方法一致性良好,可用于临床实验室曲霉体外药敏检测。Objective To evaluate the consistence of Sensititre YeastOne colorimetric panel and the reference broth microdilution method in in vitro antifungal susceptibility testing(AFST)of Aspergillus.Methods In vitro AFST of 307 Aspergillus isolates were performed by reference CLSI M38-A2 method and Sensititre YeastOne YO10 panel parallelly,and the statistical parameters including essential agreement(EA),category agreement(CA)and error rates between these two methods in Aspergillus AFST were calculated.Results 98.4%(302/307)of Aspergillus isolates were wild type(WT)against tested six antifungal agents revealed by CLSI M38-A2 AFST,while Sensititre YeastOne detected 97.1%(298/307)isolates as totally WT.The EA regarding tested antifungals were over 90%between CLSI M38-A2 method and Sensititre YeastOne in this study except for Itraconazole.The CA of these two methods in Aspergillus AFST was 98.1%(301/307);the very major error(VME)rate and the major error(ME)were 0.3%(1/307)and 1.6%(5/307),respectively.Conclusion Sensititre YeastOne has a satisfied agreement with CLSI reference method in in vitro AFST of Aspergillus,which provides a prospect of applying Sensititre YeastOne into routine AFST of Aspergillus in clinical laboratories.

关 键 词：曲霉 抗真菌药物敏感性试验 Sensititre YeastOne 微量肉汤稀释法 

分 类 号：R379.6[医药卫生—病原生物学]