基于石墨烯和功能核酸的Ag^+荧光检测方法  

Fluorescent Detection of Ag^+Using Graphene and Functional Nucleic Acid

作　　者：吴雪琪 张立佩 曹阳 文晓刚[3] 周小红[2] WU Xue-qi;ZHANG Li-pei;CAO Yang;WEN Xiao-gang;ZHOU Xiao-hong(School of Environmental and Geographic Sciences,Shanghai Normal University,Shanghai 200234;State Key Joint Laboratory of Environmental Simulation and Pollutants Control,School of Environment,Tsinghua University,Beijing 100084;Nanhu College,Jiaxing University,Jiaxing 314001)

机构地区：[1]上海师范大学环境与地理科学学院,上海200234 [2]清华大学环境学院环境模拟与污染控制国家重点实验室,北京100084 [3]嘉兴学院南湖学院,嘉兴314001

出　　处：《生物技术通报》2020年第8期210-216,共7页Biotechnology Bulletin

摘　　要：基于核酸适配体错配结合Ag^+的原理,利用石墨烯对单链DNA和双链DNA结合力的不同,设计开发了C-Ag^+-C和石墨烯结合的新型荧光传感器,并建立了一种高灵敏度、快速、便捷的荧光检测方法,实现了turn on形式的Ag^+检测。在优化条件下,检测Ag^+的线性范围为150-400 nmol/L,检出限为23 nmol/L。该方法在饮用水的实验样品中Ag^+加标检测的回收率达到96.6%,具备用于实际样品分析的能力。Based on the principle of mismatched binding Ag^+of nucleic acid aptamer,using the different binding forces of graphene to single-stranded DNA and double-stranded DNA,a new fluorescent sensor with C-Ag^+-C and graphene binding was designed and developed.A high sensitivity,fast and convenient fluorescence detection method was developed to achieve the Ag^+detection in turn on form.Under the optimization of detection conditions,the turn on fluorescent aptasensor offered a linear detection range of 150-400 nmol/L with a detection limit of 23 nmol/L for Ag^+.The recovery rate of the method was 96.6%for Ag^+-labeled detection in the experimental samples of drinking water,showing the ability to be used for actual sample analysis.

关 键 词：银离子 碱基错配 石墨烯 荧光检测 

分 类 号：O657.3[理学—分析化学]