秋水仙碱对猕猴桃愈伤组织分化的影响  

Effect of Colchicine on Tissue Differentiation of Kiwifruit

作　　者：龙自立[1] 张慧琴[2] 刘永立[1] LONG Zi-li;ZHANG Hui-qin;LIU Yong-li(College of Agriculture&Biotechnology,Zhejiang University,Hangzhou,Zhejiang 310058;Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang 310021)

机构地区：[1]浙江大学农业与生物技术学院,浙江杭州310058 [2]浙江省农业科学院园艺研究所,浙江杭州310021

出　　处：《安徽农业科学》2020年第16期49-51,共3页Journal of Anhui Agricultural Sciences

基　　金：浙江省农业新品种选育重大科技专项“猕猴桃新品种选育”(2016C02052-7-2);玉环市科技局项目“猕猴桃抗病砧木培育及应用推广”。

摘　　要：[目的]探讨秋水仙碱对猕猴桃愈伤组织分化的影响。[方法]利用组织培养技术,研究秋水仙碱的不同浓度和处理时间对愈伤组织分化率和不定芽数量的影响。[结果]秋水仙碱对猕猴桃愈伤组织不定芽分化率有很大的影响。阔叶猕猴桃愈伤组织在秋水仙碱0.05%下处理24 h,其不定芽分化率达100%,而对照为58.33%。在0.50%秋水仙碱浓度下处理24 h,海沃德愈伤组织的不定芽分化率达到最高为75%。金桃胚乳愈伤组织不定芽分化率达到最大时的秋水仙碱浓度为0.20%,处理时间为24 h。[结论]秋水仙碱处理对猕猴桃再生芽数的影响因猕猴桃种类而异,海沃德再生芽数的最佳组合:0.20%的秋水仙碱处理24 h;阔叶猕猴桃再生芽数的最佳组合:0.05%的秋水仙碱处理24 h。[Objective] To investigate the effect of colchicine on the differentiations of callus of kiwifruit.[Method]The effects of different concentrations and treatment times of colchicine on callus differentiation rate and number of adventitious buds were studied.[Result]Colchicine had a great effect on the rate of adventitious bud differentiation in kiwifruit.The rate of adventitious bud differentiation of Actinidia latifolia was 100% by 0.05% colchicine treatment for 24 hours,while the control was 58.33%;treated with 0.50% colchicine for 24 hours,the rate of adventitious bud differentiation of Hayward callus reached 75%.The differentiation rate of Jintao adventitious buds reached the maximum by 0.20% colchicines treatment for 24 hours.[Conclusion]The effect of colchicine treatment on the regeneration bud number of kiwifruit varied with kiwifruit species.The best combination of Hayward regeneration bud number was colchicine concentration of 0.20% for 24 h;the best combination of Actinidia latifolia regeneration bud number was 0.05% colchicine for 24 h.

关 键 词：猕猴桃 愈伤组织 秋水仙碱 诱导 不定芽 

分 类 号：S663.4[农业科学—果树学]